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CIAN Leica SP8 – short instructions, version 3.1, September 2015 

page 1 of 10

 

 

SHORT INSTRUCTIONS FOR OPERATING Leica SP8 AT CIAN 

Version 3.1, September 2015 

 

1 - Equipment Setup  

Fig 1

: Basic components of the Leica SP8 microscope 

1: 

Leica DMI 6000B 

inverted microscope 

2:  Scan head 
3:  Anti-vibration table 

4:  EL6000

 metal halide lamp for WFF

 

5: 

CTR control box

 for microscope stand 

6: 

Smart Move

 joystick 

7:  Monitor 

8:  Control panel 
9:  Keyboard 
10:  Computer mouse 
11:  Computer table 
12:  Lasers in 

Compact Supply Unit (CSU)

 

13:  Supply Control 
14:  Workstation 

 
 

Fig 2

: Spectral detection system, schematic 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Emission 

Light 

Prism 

Sliders 

 

PMT 

 

PMT 

 

PMT 

 

HyD 

 

Summary of Contents for SP8 AT CIAN

Page 1: ...oscope 1 Leica DMI 6000B inverted microscope 2 Scan head 3 Anti vibration table 4 EL6000 metal halide lamp for WFF 5 CTR control box for microscope stand 6 Smart Move joystick 7 Monitor 8 Control pane...

Page 2: ...t the condenser or objectives Initialization is needed for Tiling and Mark Find features 7 Visually inspect and clean objectives see section 2 2 as needed 2 1 Adjusting K hler Illumination 1 Insert a...

Page 3: ...ue fluid on the lens paper Wipe the lens gently by holding either end of the wet lens tissue dragging it gently across the objective lens three times Use a fresh area of the tissue each time Repeat wi...

Page 4: ...mode by clicking the Laser icon Activate the lasers you need by clicking on their on off button The standby feature is not required 3 2 3 Useful settings in the configuration tab Customize the USB Co...

Page 5: ...ion 1 Click SEQ in the Acquisition Mode to open the Sequential Scan control window 2 Set light path configuration for the first sequence as described above in 3 2 4 a 3 Click in the Sequential Scan co...

Page 6: ...space Rotation of the scan field by up to 100 degrees in either direction as with zoom use slider numerical factor or control panel knob Panning with the arrows positions the scan area when zoomed in...

Page 7: ...positioning during the stack Note Z Wide refers to z control via the objective focus drive 4 Zoom into cartoon view to visualize schematic stack representation 5 Z step size or number of steps can be...

Page 8: ...ect it in the Positions list 5 Using the buttons marked positions can be a Saved and reloaded b individually deleted c all deleted trash can d edited go to position using positions list adjust xyz pre...

Page 9: ...orage You are responsible for safe and redundant storage of your data on your own storage devices 1 Save projects in LAS X software to your folder on the D drive 2 Connect to the server Run command co...

Page 10: ...1 water HC PL APO 40x 1 10 W CORR CS2 DIC 5 40x 1 3 oil HC PL APO 40x 1 30 Oil CS2 DIC 6 63x 1 4 oil HC PL APO 63x 1 40 OIL CS2 DIC Confocal mode Lasers no fluorescence emission filters all spectral...

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