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CONTROLLED COPY
Centre for Biological Engineering
Standard Operating Procedure
SOP194
Title: Use and maintenance of Leica RM2125 RTS rotary microtome.
Location:
H27
Version 001
Effective Date: 24.05.2021
Review 24.05.2023
Written by: Sotiria Toumpaniari
Reviewed by:
Approved by: C.Kavanagh
Page 1 of 11
1. PURPOSE
To describe the use and maintenance of the Leica RM2125 RTS rotary microtome that is located in the
CL2 CBE laboratory H27.
2. SCOPE
This SOP applies to CBE personnel using the Leica RM2125 RTS rotary microtome for the sectioning
of fixed soft tissues that have been embedded in wax or other hard polymers. The microtome is
assigned to H27 which is used for histological processing and this system should not be transferred to
another lab. Only authorised and trained personnel may use the microtome.
3. RESPONSIBILITES
3.1. Authorised laboratory staff shall:
(i)
Work in accordance with the instructions for operation and maintenance detailed in this SOP,
Risk Assessment and the operator manual.
(ii) Carry out the routine inspection, cleaning, and maintenance of the equipment, as required.
(iii) Record any adverse events and alarms that indicate non-conformance or malfunction on the
Maintenance Record and notify the Laboratory Manager/Responsible Person.
3.2. The Responsible Person/Laboratory Manager shall:
(i) Ensure that authorised laboratory personnel are given suitable information, instruction, training
and supervision in the correct use and maintenance of the equipment.
(ii) Investigate any reported problems, adverse event, alarms, or non-conformities associated with
equipment usage.
(iii) Organise the maintenance, repair, or servicing of the equipment by trained and authorised
contract / service personnel.
4. EQUIPMENT AND MATERIALS
1. Leica RM2125 RTS rotary microtome and accessories
2. Blades
3. Brush
4. Appropriate PPE (labcoat, gloves, overshoes and eye protection, where appropriate)