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    56-2853-00 AE, 2002-12 • p1

inst

ructions

    Gradient Mixer GM-1

1. Introduction

The Gradient Mixer GM-1 (120 V, Code No. 19-0495-01,
230 V Code No. 19-0485-01) consists of two identical
reservoirs mounted on a base plate; connection between the
reservoirs is made via a valve. Mounted above the mixing
reservoir is a motor unit which drives a stirrer blade. The
outlet from the mixing reservoir to the column is fitted with
a flow control valve, FCV-1. The Gradient Mixer is
conveniently supported on a laboratory stand. Capillary
tubing, 2 m, is also supplied.

2. Safety

Warning!

When using hazardous chemicals, all suitable protective
measures such as protective glasses, must be taken.

3. Unpacking

Unpack the equipment carefully and check the contents of
the carton against the packing list.

4. Description

THE BASE PLATE (11)* is a precision machined
polypropylene block on which the reservoir cylinders (7) are
mounted.

Sealing between the base plate and the cylinders is provided
by O-rings (8).

THE RESERVOIR CYLINDERS (7) are straight
polymethylpentene tubes threaded at one end and with air
inlets at the other end. Each cylinder has a capacity of 300 ml
and is graduated to 250 ml at 25 ml intervals to give a rapid
check of the volume of liquid remaining in the reservoirs.
The mixing reservoir carries the motor unit (1, 2) and the
other reservoir is fitted with a snap-on lid (3).

THE MOTOR UNIT (1, 2) consists of a fully encapsulated
low speed 250 or 300 rpm synchronous motor mounted in
polypropylene housing. A phasing condensor ensures that
the direction of stirring is always the same and that the

stirrer blade (6) lifts the liquid entering the mixing reservoir.
The motor unit is supplied for operation from 100–120 or
220–240 V AC, 50 or 60 Hz.

THE STIRRER BLADE (6) is secured to the motor axle by a
nylon screw (5). It is unnecessary to remove the screw to
release the stirrer blade.

THE CONTROL VALVE (12) regulates the flow of liquid
between the two reservoirs. It is fully open when screwed out
one complete revolution from the closed position.

THE FLOW CONTROL VALVE (18) is a needle valve
designed for shut-off as well as flow control. The valve
nipple accepts capillary tubing 1.6–1.9 mm o.d. Eluent flow
is regulated with the large knob (A). The line on this knob
and the markings on the valve housing can be used as a flow
rate guide. The valve can thus be opened to a previously
determined position to obtain a given flow rate. To obtain
reproducible flow rates the valve should always be returned
to the shut position before setting.

THE STAND MOUNT (21) may be used to support the
Gradient Mixer on a laboratory stand. When not required,
it may be removed from the base plate (11) by unscrewing
the retaining knob (20).

5. Installation

Connection of tubing to the flow control valve

1. Cut the tubing cleanly at a 45° angle.

2. Remove the screw cap and slip it over the tubing.

3. Remove the rubber cone with the help of the metal

stopper.

4. Push the tubing through the rubber cone until it projects

about 1 cm.

5. Seat the tubing in the nipple. Slide the cone along the

tubing, into the nipple.

6. Finger tighten the screw cap.

* Numbers refer to the enclosed Spare Parts drawing.

Summary of Contents for GM-1

Page 1: ...lypropylene housing A phasing condensor ensures that the direction of stirring is always the same and that the stirrer blade 6 lifts the liquid entering the mixing reservoir The motor unit is supplied for operation from 100 120 or 220 240 V AC 50 or 60 Hz THE STIRRER BLADE 6 is secured to the motor axle by a nylon screw 5 It is unnecessary to remove the screw to release the stirrer blade THE CONTR...

Page 2: ...act shape of the pH gradient obtained will depend on the buffer salts used and the ionic strengths and pH s of the buffer solutions The use of gradient elution in ion exchange chromatography is described in the booklet Ion Exchange Chromatography principles and methods 7 Maintenance Warning When using hazardous chemicals all suitable protective measures such as protective glasses must be taken Cle...

Page 3: ...e 19 Sealing plug 19 0039 01 E 5 20 Retaining knob 19 0489 01 G 1 21 Stand mount 19 0463 01 H 1 22 Tubing 19 0040 01 I 10 m 23 Stopper 19 0752 01 H 10 material of main component Materials A Polypropylene B Polyvinylchloride C Polyamide D Polymethylpentene TPX09 E Fluororubber F Polyurethane G Superpolyoxymethylene H Stainless steel I Polyethylene 9 Spare parts Caution Only spare parts approved or ...

Page 4: ...u ku Tokyo 169 0073 Japan All goods and services are sold subject to the terms and conditions of sale of the company within the General Electric Company group that supplies them A copy of these terms and conditions is available on request Meaning Consult the instruction manual to avoid personal injury or damage to the product or other equipment Warning The Warning sign is used to call attention to...

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