BrandTech seripettor Operating Manual Download

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Liquid Handling · Easy Handling!

Gebrauchsanleitung

F I R S T

C L A S S · B R A N D

Operating Manual

Mode d'emploi

Instrucciones de manejo

Istruzioni per l'uso

seripettor

Summary of Contents for seripettor

Page 1: ...Liquid Handling Easy Handling Gebrauchsanleitung F I R S T C L A S S B R A N D 3 Operating Manual 27 Mode d emploi 51 Instrucciones de manejo 75 Istruzioni per l uso 99 seripettor ...

Page 2: ...ing 36 Dispensing with flexible discharge tube 37 Error Limits Nominal Volume Partial Volume 38 Checking the Volume Calibration 39 Cleaning 40 Cleaning replacing valves 41 Maintenance 43 Replacing the dispensing cartridge 43 Autoclaving 44 Preparations for sterile operation 44 Ordering Information Accessories Spare Parts 45 Troubleshooting 48 Repairs Contact Addresses 49 Warranty Information Dispo...

Page 3: ...son is endangered When dispensing the discharge tube must always point away from you or any other person Avoid splashes Only dispense into suitable vessels 7 Never press down the piston when the dis charge tube closure screw cap is attached 8 Secure the piston lock during operation 9 Never remove the discharge tube while the dispensing cylinder is filled 10 Reagents can accumulate in the closure c...

Page 4: ...kinematic viscosity 2 ml instrument 300 mm2 s 10 ml instrument 150 mm2 s 25 ml instrument 75 mm2 s dynamic viscosity mPas kinematic viscosity mm s x density g cm density up to 2 2 g cm3 Limitations of Use Operating Limitations Liquids which form deposits may accelerate wear on the piston seal and make the piston difficult to move or may cause jamming e g crystallizing solutions or concentrated alk...

Page 5: ...concentrated HCl Polar solvents such as acetone UV sensitive reagents Store the instrument and accessories only in cleaned condition in a cool and dry place Storage temperature from 20 C to 50 C to 4 F to 122 F Storage Conditions seripettor never use with liquids attacking FEP PP PE or EPDM non polar solvents like hydrocarbons and halogenated hydrocarbons concentrated or oxidizing acids explosive ...

Page 6: ...ric acid 10 Butanediol 1 Butanol Butylamine n Butyl acetate Calcium carbonate Calcium chloride Calcium hydroxide Calcium hypochlorite Chloroacetaldehyde 45 Chloroacetic acid Chromic acid 50 Copper sulfate Cumene Isopropyl benzene Diethylene glycol Dimethyl sulfoxide DMSO Dimethylaniline Ethanol Formaldehyde 40 Formamide Formic acid 100 Glycerol Medium seripettor seripettor pro Glycol Ethylene glyc...

Page 7: ...Elements Pump assembly Piston lock Volume adjustment Dispensing cartridge piston cylinder Valve block Discharge tube Stopper cap Filling tube Discharge tube with integrated valve Adapter for discharge tube Screw cap ...

Page 8: ...e 2x dispensing cartridge 3x bottle adapters PP and this operating manual seripettor pro Pump assemblx seripettor pro valve block with discharge tube pre assembled telescoping filling tube 2x dispensing cartridge 3x bottle adapters PP mounting tool and this operating manual 2 Mount pump assembly Screw dispensing cartridge into valve block Fig 1 1 Unscrew dispens ing cartridge a Open piston lock on...

Page 9: ...ion Screw the instrument GL 45 thread onto the reagent bottle fig 4 To prevent tipping over use a bottle stand for small bottles 3 Mounting the filling tube seripettor Cut off the filling tube to accommodate the bottle height and attach it fig 3 seripettor pro Adjust the length of the telescopic filling tube to the bottle height and attach it fig 3 Always wear protective gloves when touching the i...

Page 10: ...p out from the discharge tube and stopper cap Warning Assembly cont 6 Priming the instrument a Hold the discharge tube and loosen the stopper cap screw cap fig a b To avoid splashes hold the discharge tube orifice on the inner wall of a suitable receiving vessel Allow the piston to rise up approx 30 mm and push it down rapidly until the lower stop fig b Repeat this procedure approximately 5 times ...

Page 11: ...n the stopper cap screw cap b Hold the discharge tube orifice on the inner wall of a suitable receiving ves sel c Press the piston down slowly and steadily without using excessive force to the lower stop Then let it rise up slowly fig c d Wipe off the discharge tube against the inner wall of the receiving vessel e Reattach the stopper cap to the discharge tube Wear protective clothing eye protecti...

Page 12: ...inks or the like Each time you are going to use the tubing examine it carefully To dispense aggressive liquids you should take safety mea sures in addition to the normal precautions We recommend use of a protective shield The bottle must be supported using a bottle stand To help avoid reagent splashing from the tube always grip the tube firmly by the handle and replace into the holder after use Fo...

Page 13: ...2 4 10 1 2 120 0 2 20 25 1 2 300 0 2 50 A Accuracy CV Coefficient of Variation Error limits seripettor seripettor pro 20 C Ex 38 e g Volume A µl CV µl VN 10 1 2 120 0 2 20 VT 50 N 5 2 4 120 0 4 20 VT 10 N 1 12 120 2 0 20 Note The maximum error limit for a single measurement can be calculated EL A 2 CV e g for volume 10 ml 120 µl 2 x 20 µl 160 µl Partial volume The percentage values for A and CV ar...

Page 14: ... mg at 20 C 1013 hPa V0 nominal volume Mean volume V V0 V0 A 100 Σ xi x 2 n 1 s Z Mean value x Σ xi n Mean volume V x Z Accuracy Standard deviation Coefficient of variation 100 s V CV 1 Preparation of the instrument Clean the instrument Cleaning page 40 fill it with distilled H2 O and then prime it carefully 2 Check the volume a 10 dispensing operations with distilled H2 O in 3 volume ranges 100 5...

Page 15: ...he dispensing cylinder is filled Point the valves and tube openings away from your body Wear protective clothing eye protection and appropriate hand protection Warning immediately when the piston is difficult to move before changing the reagent prior to long term storage prior to dismantling the instrument prior to changing the valve The instrument must be cleaned in the following situations to as...

Page 16: ...ntaminated individual parts e g in an ultrasonic bath e Insert cleaned or new sealing ring f Screw in the clean or replacement filling valve first by hand and then tighten it with a coin fig 1f 2 Discharge valve a Loosen the discharge tube locking nut b Remove the discharge valve c If the sealing ring is contaminated or damaged carefully remove it with a pair of curved forceps d If necessary clean...

Page 17: ... b Use the mounting tool to unscrew the filling valve fig 1b c If the sealing ring is contaminated or damaged carefully remove it with a pair of curved forceps fig 1c d If necessary clean any contaminated individual parts e g in an ultrasonic bath e Insert cleaned or new sealing ring f Screw in the cleaned or replacement filling valve first by hand and then tighten it with the mounting tool fig 1f...

Page 18: ... and empty it com pletely with several dispensings 2 Manually press the pump assembly all the way down and hold it there Loosen the volume selector thumb screw one half turn slide the pointer vertically to the upper stop and then retighten the volume selector thumb screw fig 2 3 Loosen the piston lock fig 3 4 Unscrew and remove the pump assembly fig 4 5 Unscrew the dispensing cartridge fig 5 and r...

Page 19: ...7 4 Loosely screw the closed valve block with the filling tube and discharge tube onto the filled bottle 5 Close the air vent opening with an autoclavable membrane filter 0 2 µm fig 5 6 Open the discharge tube and loosen the locking nut fig 6 7 Autoclave After autoclaving the instrument should be used only after it has reached room temperature Exception seripettor with agar culture media up to 60 ...

Page 20: ...n Bottle adapters PP For seripettor and seripettor pro Pack of 1 Description Pack of Cat No 2 ml non sterile 3 7045 00 10 ml non sterile 3 7045 02 25 ml non sterile 3 7045 04 2 ml sterile 7 7045 07 10 ml sterile 7 7045 06 25 ml sterile 5 7045 08 Dispensing cartridges For seripettor and seripettor pro Non sterile and sterile Piston PE cylinder PP Note Dispensing cartridges are not autoclavable indi...

Page 21: ...No 2 10 ml 6697 25 ml 6698 Filling valve seripettor pro Filling valve with sealing ring Pack of 1 Sealing rings seripettor Pack of 5 EPDM Cat No 6788 Description Cat No for discharge tube 1 PP 6208 for filling valve 2 ETFE 6707 Adapter seripettor pro With sealing ring PTFE Pack of 1 Discharge tube adapters are ordered separately not suitable for peroxides Description Cat No 2 10 ml 7045 22 25 ml 7...

Page 22: ... ml 7045 48 25 ml 7045 49 Pump assembly seripettor pro PPO PEI UV protec tion Hastaloy stain less lifting spring Pack of 1 Description Cat No 2 10 ml 6792 25 ml 6794 Valve block For seripettor and seripettor pro Pack of 1 Description Cat No 2 10 ml 7045 52 25 ml 7045 54 Cap for closing valve block For seripettor and seripettor pro Pack of 1 Description Length Cat No 2 10 ml 70 140 7042 02 125 240 ...

Page 23: ...y Slowly draw in reagent The instrument has not been primed Prime the instrument page 35 Dispensing cartridge worn Replace dispensing cartridge page 43 Filling tube not firmly con nected or damaged Cleaning procedure page 40 Push the filling tube on firmly If necessary cut off approx 1 cm of tube at the upper end and re connect it or replace filling tube Valves not firmly connected contaminated or...

Page 24: ...the Return Autho rization Number prominently displayed on the outside of the package to the address provided with the Return Authorization Number BRAND GMBH CO KG Otto Schott Straße 25 97877 Wertheim Germany Tel 49 9342 808 0 Fax 49 9342 808 98000 E Mail info brand de www brand de USA and Canada BrandTech Scientific Inc 11 Bokum Road Essex CT 06426 1506 USA Tel 1 860 767 2562 Fax 1 860 767 2563 ww...

Page 25: ...horized repairs of the instrument or the consequences of normal wear and tear especially of wearing parts such as pistons seals valves and the breakage of glass as well as the failure to follow the instructions of the operating manual We are not liable for damage resulting from any actions not described in the operating manual or if non original spare parts or components have been used U S and Can...

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