Plateforme de Microscopie Photonique
Page 2 of 2
Updated on 2019-02-12
User Guide Zeiss Z2 B-301
Setup
On the microscope touch screen:
9.
Press Home>
Load Position
10.
Press
Set Work Position
11.
Move the focus slightly upward to remove the «
Lower Z limit reached
» message
12.
Press Home>Microscope>Turret>
Objectives
>
10x
13.
Press
Done
(if prompted)
14.
Press Home>Microscope>XYZ>Position>
Set zero Auto
(focus calibration)
15.
Press
OK
16.
Wait until the calibration is done
Loading samples
On the microscope touch screen:
1.
Press Home>
Load Position
2.
Press
Set Work Position
On the microscope:
3.
Place your sample on the microscope stage
Focusing with air objectives
On the microscope touch screen:
5.
Press Home>Microscope>Turret>
Objectives
6.
Press
10x, 20x
OR
40x
On the computer:
7.
Open
Zen Blue
software
8.
In the
Locate Tab
, select
BF Visible
or
Fluo Visible
9.
Adjust the focus while looking through the eyepieces (focus is reached around Z=13mm on a calibrated focus)
10.
In the
Locate Tab
, select
Off
Focusing with oil objectives
Focus first with an air objective
then, on the microscope touch screen:
8.
Press Home>Microscope>Turret>
Objectives
9.
Press
60x, 100x 1.3
OR
100x 1.4
10.
Place a drop of oil on your sample
11.
Press
Done
In
Zen Blue
software:
12.
In the
Locate Tab
, select
BF Visible
or
Fluo Visible
13.
Adjust the focus with the fine adjustment wheel while looking through the eyepieces (focus is reached around Z=13mm on
a calibrated focus)
11.
In the
Locate Tab
, select
Off
Your sample is ready for acquisition.
