Zeiss LSM 880, Operating Manual

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CHAPTER 5 - MULTIPHOTON LASER SCANNING …
ZEISS Non Descanned Detection (NDD) LSM 880
22 000000-2071-464 10/2014 V_01
2.5 Changing the Filter Modules BiG.2
You can remove and replace filter modules by push and click.
When opening the filter insert, the safety control will switch off the laser light.
To exchange the filter module of the BiG.2 proceed as follows:
•
Loosen the two knurled screws (Fig. 17/
2
).
•
Remove the filter insert (Fig. 17/ 3 ) of the BiG.2 (Fig. 17/ 1 ) and put it down.
How to uninstall a module:
•
Disengage the filter module (Fig. 18/
4
) from the upper spring elements (Fig. 18/
2
) of the filter insert
(Fig. 18/ 5 ) by tilting it forward, then lift it off the lower spring elements (Fig. 18/ 1 ) and remove the
module.
How to install a module:
•
Insert the filter module (Fig. 18/ 4 ) with the aid of its mounting elements on its left and right (
318H
Fig. 18/ 3 )
into the lower spring clips (
319H
Fig. 18/ 1 ) on the filter insert.
•
Press the filter module against the upper spring clips (
319H
Fig. 18/ 2 ) until it engages firmly.
•
After exchanging the filter module, replace the filter insert (Fig. 17/ 3 ) into the BiG.2 (Fig. 17/ 1 ).
•
Fasten the two knurled screws (Fig. 17/
2
).
Fig. 17 Filter insert of the BiG.2 Fig. 18 Changing the filter module
Once the NDD modules are connected and the filters are placed in front of the detectors, use the
Filters
tool in the Maintain tab to indicate the filters to the software. See section See section 5.5 Maintain Tab
in CHAPTER 1: SYSTEM OPERATION. The shorter emission wavelength is registered by channel 1
(BiG Ch1) and the longer wavelength is registered by channel 2 (BiG Ch2).