Zeiss LSM 880, Operating Manual

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CHAPTER 5 - MULTIPHOTON LASER SCANNING …
LSM 880 NLO (Non Linear Optics) Laser for LSM ZEISS
10/2014 V_01 000000-2071-464 5
1.3 Motorized Periscope
•
Systems with OPO or two lasers coupled into
one scan head are equipped with a motorized
periscope. The interface for controlling the
motorized mirrors within the periscope is in the
Maintain tab/ System Options tool. Scroll to
the Motorized Periscope tab in the tool (Fig. 3).
A pair of mirrors is related to each one of the
lasers or to the OPO. Input addresses the mirror
at the entry of the periscope. Output addresses
the mirror at the exit of the periscope next to
the scan head. For each mirror two axis can be
controlled via the arrows in the corresponding
line. Corrections should only be done in small
steps and should only be necessary for
controlling the overlay of the two beams if
beams are to be used for the excitation of the
same area simultaneously or sequentially. If the
beam is lost by operating the arrows it might be
necessary to get a service engineer for
completely realigning the beam.
•
Optionally the periscope can be equipped with
photosensitive diodes (auto-alignment function)
providing the function of resetting the beam to
the preset positions. The optimal position for
the overlay of the two beams is set by the
service engineer at installation. In the
Channels
tool a button is present ( Align active Beams ,
Fig. 4). Clicking the button will move the
mirrors such that the active beam(s) is/are again
at this predefined position.
Fig. 3 Motorized Persicope panel
Fig. 4 Channels tool with laser and OPO
activated showing the Align active
Beams button