Zeiss LSM 880, Operating Manual

Page: 307 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 301
5.4.4 Processing – Color-coded Projection
Licensing Instrument

standard optional

LSM

ELYRA

Lightsheet Z.1
Tool accepts Z-stacks and time series (incl. combinations with several other
dimensions). See full description of tool for details.
The
Color-coded projection function generates a
maximum intensity projection image along the z-,
or time- dimension of a multidimensional data set
(Fig. 398). Instead of using the colors assigned to
the channels, it displays the position in z or in time
with a color gradient.
•
To open the
Color-coded projection
Processing tool, select it from the list in the
Processing main tab.
•
Click on the triangle buttons to show or hide
the
Method Parameters and Preview panels
of the function.
•
Select the color gradient by choosing an
appropiate look up table (LUT) from the
LUT
drop down menu.
•
You can select from the
dimension
drop down
menu any dimension of the image. The color
gardient will apply for the selcted dimension.
These include
Z
(for a Z-Stack) or
T
(for a time
series).
•
Below the color gradients the corresponding
depth or time is indicated in grey numbers and
letters.
•
Define the range (
ROI
) of the Z-Stack or time
series to be used by using the sliders or the
input boxes with arrows.
•
Clicking the
Apply
button creates the color-
coded projection as a new image document
using the name shown in the Output image
display for the Select field.
Fig. 398 Processing – Color-coded projection