Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
246 000000-2071-464 10/2014 V_01
To compensate for scan zoom and scan speed go
to the
Acquisition Mode
too of the LSM
Online
Acquisition tool group. Use a sample with high
contrast, like "Convolaria", and focus. Activate
Bidirectional Scan (double arrow) under
Direction
(Fig. 328). During scanning press the
Auto button. The system will adjust automatically
the
Scan Corr X
and
Y
values. Alternatively you
can do the compensation manually.
If the Scan Corr is not adjusted, you will see a
pixel shift (Fig. 329). Alter the Scan Corr values to
compensate any pixel shift in the following way:
Move the
Scan Corr X
slider until the pixel shift is
at the minimum (Fig. 330). Rotate the image by
90° and redo the same adjustment for
Scan
Corr Y
.
Fig. 328 Pixel Depth, Scan Direction & Scan
Average window
Fig. 329 Image of a line before pixel shift
compensation in x
Fig. 330 Image of a line after pixel shift
compensation in x