Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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The X-, Y- and Z-coordinates of the selected positions are only stored in the
Positions
table and
not in the scanned image (stack). Accordingly, all the marked positions in all Z-planes are displayed
in the scanned image (Z-Stack), no matter in which Z-plane they are.
•
When finished, click on the
Snap
or
Start Experiment
action button to start the FCS measurements
at the highlighted or all listed positions, respectively. The measurement procedure starts and the
Image FCS diagram window appear in a container.
(4) Hysteresis correction of Scanning mirrors
In general, there is no need to compensate between
defined positions and the actual measurement positions
using the scanning mirrors.
However, the precise arrest position of the mirrors
depends on the used scan zoom and speed and small
offsets can occur and offsets may exist due to scanner
hysteresis (Fig. 326).
To adjust for differences, the compensation sliders of the
bidirectional scan can be adjusted to decrease the offset
(Fig. 327).
Fig. 326 Scanner positioning before
hysteresis correction
Fig. 327 Scanner positioning after
hysteresis correction