Zeiss LSM 880, Operating Manual

Page: 225 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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Translation
You have the following possibilities for setting
parameters (Fig. 286):
•
You can either fit to fractions (used normally
when no brightness differences are observed
between different components) or to fractional
intensities, by unticking or ticking the
Fractional Intensities
option check box
respectively. In the latter case, you have to
provide the absolute or relative brightness
values of the components, which are entered
into the
Molecular brightness
selection boxes.
•
You can either fit to the diffusion time, or you
can directly fit to the diffusion coefficient by un-
ticking or ticking the
Diffusion coefficients
check box, respectively. In the latter case you
have to provide the dimension of the radius of
the confocal volume in the
ω
r
selection box.
Either type in a value or use the arrow keys.
•
You must also specify if you use two photon
excitation by checking the
2 Photon check box,
if since that will influence the fit formula in the
case the
Diffusion coefficients option was
chosen.
•
You can select the numbers of components
(1, 2 and 3) by pressing the
Components 1
,
2
or 3 buttons. The number of active components
will be highlighted in blue.
•
For each component you can select free or anomalous diffusion in the pull down menus of the
Free/anomalous
selection box.
•
For each component you can set the dimensionality of diffusion in the pull down menus of the
Dimension selection boxes. You can toggle between 1-D , 2-D and 3-D .
•
For each component you can enter brightness values in the
Brightness
select boxes. These values are
only displayed, if the Fractional Intensities option is selected. You can type in absolute values (that
must have the same units) or relative values.
The description box gives information on the translational diffusion terms.
Fig. 286 Translation Settings panel