Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
138 000000-2071-464 10/2014 V_01
5.2.14 Acquisition Parameter – Regions
The Regions tool tab is only available, if the
Regions
checkbox is ticked in the
Multidimensional acquisition field. If the tool bar is
expanded, the
Regions tool becomes available
(Fig. 185).
The
Regions tool allows the user to define Regions
of Interest (ROI) which are used for display for
WF
,
SIM and Laser WF imaging modes as well as
image acquisition, sample manipulation (bleaching)
and image analysis for
LSM imaging mode.
The control of the laser in
LSM imaging
mode via
AOTF technology enables the restriction of the
illumination to the defined region. The sample is
only illuminated when data are acquired. In
widefield techniques (
WF
,
SIM
,
LSM WF
), the
whole sample is always illuminated, even if only
the region is displayed.
The definition of the regions is done using an
actual image of the sample and drawing the
regions into this image (Fig. 186, Fig. 187). This
makes it extremely easy to set the regions in the
right position and dimension.
Fig. 185 Regions tool
Fig. 186 Image with two defined regions,
the square one is selected
Fig. 187 Regions tool