8
03/06
Configuring the beam path and lasers
•
Click on the
LSM
button in the
Acquire
subordinate toolbar for laser scanning.
Choosing the configuration
Single Track
−
Use for
single
, double and triple labeling;
simultaneous scanning only
−
Advantage: faster image acquisition
−
Disadvantage: cross talk between channels
Multi Track
−
Use for double and triple labeling; sequential
scanning, line by line or frame by frame
−
Advantage: when one track is active, only
one detector and one laser is switched on.
This reduces cross talk.
−
Disadvantage: slower image acquisition
•
Click on the
Config
button in the
Acquire
subordinate toolbar to
open the
Configuration Control
window.
The
Configuration Control
window
appears (Fig. 12).
Setting for single track configuration in Channel Mode
•
Select
Channel Mode
if necessary (Fig. 12).
•
Click on the
Single Track
button in the
Configuration Control
window (Fig. 12).
•
Click on the
Descanned
button if necessary.
The
Beam Path and Channel Assignment
panel of the
Configuration Control
window displays the
selected track configuration which is used for the scan procedure.
•
You can change the settings of this panel using the following function elements:
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider). Open the
Laser Control
window via the
Laser
button.
Selection of the main dichroic beam splitter (HFT) or secondary dichroic beam splitter
(NFT) position through selection from the relevant list box.
Selection of an emission filter through selection from the relevant list box.
Activation / deactivation (via check box) of the selected channel (Ch 1-4, monitor diode
ChM, META detectors ChS1-8, transmission ChD) for the scanning procedure by
assigning an existing color icon or defining a new one.
Fig. 12
Configuration Control window for
Single Track
