LSM 510
ANNEX
LSM 510 META
Beam Path Configuration Guide for Fluorescence Microscopy ...
Carl Zeiss
03/06
B 45-0021 e
8-3
8.1.1.4 Miscellaneous
•
Plates
do transmit light 100%. They are used for a correct beam guidance and will be set
automatically.
•
Mirrors
do deflect 100% over the whole spectral range and can be used to guide the emission light
to selected detectors.
8.1.2
Setup of Single Tracks Using Single Detectors
•
Switch on the suitable lasers for excitation of the dyes in the specimen. For the UV laser and the Argon
laser set the tube current of the laser to a value of app. 50% (
Excitation, Laser, Output [%]
).
Example: for Alexa 488 and CY 3 switch on
Argon
(blue excitation) and
HeNe1 or DPSS 561
(green
excitation).
•
Activate the proper laser lines in the
Line Active
check box, set
Transmission [%]
for each active
line.
Example: Select 488 to 4% and 561 to 18%
•
Select a main dichroic beamsplitter (HFT) which deflects the selected laser lines to the specimen.
Example: HFT 488/561/633
Fig. 8-1
Configuration Control window and Excitation panel
