Zeiss Lightsheet Z.1, Operating Manual, Page: 341 / 427

CHAPTER 4 - SYSTEM OPERATION
Lightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss
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3.4.4 Maintain – Streaming
Open the Streaming tool window (Fig. 268) to activate this option by checking the box next to
Streaming . This will activate the function that data is saved and automatically written to the hard drive
during the acquisition process, so when image acquisition is disrupted, the data up to this point will be
available.
This is advisable and should be activated by default since large datasets can be acquired with the
Lightsheet Z.1 system.
If you have a storage and analysis PC you wish to stream onto, make sure that the directory is
mapped. When you turn on the PC for system control after the storage and analysis PC is
completely booted, the directory is mapped automatically.
Activated Streaming will enhance the speed by which .czi datasets are opened.
Before an Experiment is started, a Windows
explorer window will open. Here the destination of
the streaming and a file name can be chosen. The
image series will then be saved with this file name
extended by the exact date and time of the image
acquisition.
The data can be separated into separate files,
defined by chosen dimensions. More than one
dimension can be selected. As a default for later
Lightsheet Processing , Time is activated and
should remain this way. It will generate the file
structure for later Lightsheet Processing, Multiview
Processing and Online Multiview Processing.
In the
Dimension selection field, you can define how the image series is saved. For each dimension, you
can define by ticking the respective
Separate Files
checkbox to save this dimension into separate files.
The file names of the single files will have extensions of a letter indicating the dimension (t for time series,
z for Z-Stack, etc.) preceding a number that indicates the frame number from the series. If none is
checked, the whole image series will be saved into one file.
Fig. 268 Streaming tool window