Zeiss Lightsheet Z.1, Operating Manual

Page: 33 / 427

CHAPTER 1 - HARDWARE
Lightsheet Z.1 User Interfaces Carl Zeiss
02/2013 000000-1790-528 29
3.3.3 Insertion of the Drain Connector, Luer-Lock Connectors and Blind Plugs
−
Turn the assembled sample chamber body with dove tail slide so that the dove tail slide is on the
table with the grip facing you (Fig. 22/
1 ).
−
Take the grey drain connector and screw this into the upper left hole of the sample chamber body
until finger-tight (Fig. 22/
6 ). This will guide excess liquid to the drain.
−
Take a Luer-Lock-connector (white) and screw it into the lower right corner of the sample chamber
body (Fig. 22/
4
). The Luer Lock connectors (white) are used to fill or perfuse the sample chamber
with liquids and / or gasses.
−
For CO
2
incubation, the Luer-Lock connector must be positioned at the upper right corner of the
sample chamber (Fig. 22/ 2 ). For syringe based perfusion one can use any of the three lower screw
holes. The lower right corner screw hole is recommended for convenience (Fig. 22/
4
).
−
For perfusion pump use the right side bottom screw hole for the perfusion input and either of the
front lower corner screw holes for the perfusion output.
−
Close all remaining sample chamber screw hole openings by inserting the black blind plugs
(Fig. 22/
3
; Fig. 22/
5
).
1 Sample chamber body with dove tail slide
2 Luer-Lock-connector (white); position recommend for CO
2
incubation
3 Blind plug (black)
4 Luer-Lock-connector (white); position recommend for syringe based perfusion
5 Blind plug (black)
6 Drain connector (grey)
Fig. 22 Insertion of the Drain connector, Luer-Lock-connectors and Blind plugs