Zeiss Lightsheet Z.1, Operating Manual

Page: 284 / 427

CHAPTER 4 - SYSTEM OPERATION
Carl Zeiss Left Tool Area and Hardware Control Tools Lightsheet Z.1
114 000000-1790-528 02/2013
3.3.13.1 PALM – PALM
The PALM function allows setting filters for peak
finding and choosing a fit model for the
localization calculations.
Select the
PALM tab to select the PALM
Processing tool (Fig. 162). Activate the image you
want to process in the image container and press
the
Select button to load the image. Then expand
the
Method Parameters
tool window to have
access to the Peak Finder and Localizer
(Fig. 163).
You have to select the subdirectory PALM
tab in order to have the PALM functionalities
available. The directory
PALM
tab will not
give aces to the PALM tools.
Pressing the Settings Default button will reset all
filter and parameter settings to the default values.
Pressing the
Settings Reuse button will recall the
parameter setings of the actively selected image.
The
Settings
drop down menu (Fig. 164) will
allow you to define how peak events will be fitted.
You have trhe options.
−
Discard overlapping molecules
(single
emitter): only single emitters will be taken
into account and fitted, whereas all multi-
emitter events will be discarded.
−
Ignore overlap (ignore emitter): all single-
and multi-emitter events will be taken into
account and fitted as single emitter events.
−
Account for overlap (multi-emitter): multi-
emitter events wil be fitted taking into
account the overlap.
In the
Channel drop down menu you can select
the channel for which the Processing should be
conducted for a multi-channel image.
Expand the
Peak Finder
field to have access to the
peak selection filters (Fig. 165). Two filters are
available:
−
Peak mask size
−
Peak intensity to noise
Use the sliders or input spin boxes to set the
corresponding values.
Fig. 163 Processing – PALM – PALM
Fig. 164 Settings drop down menu
Fig. 165 Peak Finder Expansion field