Zeiss Lightsheet Z.1, Operating Manual

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CHAPTER 1 - HARDWARE
Carl Zeiss User Interfaces Lightsheet Z.1
24 000000-1790-528 02/2013
3.3 Assembly of the Sample Chamber
−
Wear powder-free gloves to avoid fingerprints on the cover slips as well as to protect your hands
from sharp edges.
3.3.1 Assembly of the Sample Chamber Windows
−
Assemble the three sample chamber body windows (for the illumination optics on the sides and
the front chamber window for the overview camera with LED white illumination ) using the
following steps:
1.
Take the sample chamber body (Fig. 18/ 1 ) and lay it onto the table so that one of the three smaller
windows are facing you.
2. Using a forceps or gloved hands, remove one clean circular 18 mm cover slip; place cover slip into
the empty sample chamber body window so it fits into the smallest groove (Fig. 18/
2 ) – you will
need a fine forceps.
3. Now place the O-ring (18 mm O-ring, black) into the corresponding groove (Fig. 18/ 3 ). Make sure
not to disturb the positioning of the cover slip.
4. Take the silver illumination adapter ring (Fig. 18/
4
) and lay it into the window making sure the flat
side with the four pinholes is facing you.
5. Using the sample chamber window tool (Fig. 18/
5
), position the circular pins into the pinholes and
turn clockwise until finger-tight. Adapter should be flush with the sample chamber body.
1
Sample chamber body
2
Cover slip
3
O-ring (18 mm O-ring, black)
4
Illumination adapter ring
5 Sample chamber window tool
Fig. 18 Assembly of the three sample chamber windows for illumination optics and overview
camera