Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
66
000000-1790-528
02/2013
3.2.10
Multidimensional Acquisition – Z-Stack
The
Z-Stack
tool tab is only available if the
Z-Stack
checkbox is ticked in the
Multidimensional
acquisition
field. If the tool bar is expanded, the
Z-Stack
control panel becomes available (Fig. 94).
When
Start Experiment
is pressed, the system will perform a Z-Stack.
Be aware that you might need a wider range for the Z-Stack to include enough fiducials or other
landmarks for landmark alignment processing later.
With the Z-Stack function, a series of XY-images in different focus positions can be acquired resulting in a
Z-Stack. In this way, a 3 dimensional data set from the specimen is obtained.
The way in which the Z-Stack is acquired can be selected by pressing either the
First/Last
or
Center
mode buttons. The modes differ in the way the first and last images of the stack are selected. The
Center
mode is only available in the
Show all
mode.
3.2.10.1
Performing a Z-Stack Using
First/Last Mode
•
Press the
First/Last mode
button (Fig. 94) to
open the
First/Last definition
panel.
•
Press the
Continuous
action button and focus
onto the specimen.
•
While imaging, change the focus using the
focus drive of the Ergo Drive control panel or
the z-drive control within the
Specimen
Navigator Tool tab
.
•
When you reach the position within the
specimen where you would like to start the
stack, press
Set
First
and this position will be
taken as the first position in the stack.
•
Move the focus in the other direction until the
end position within the stack is reached, then
press
Set
Last
. These focus positions are then
indicated next to
Set First
and
Set Last
buttons. The
Range
display box shows the total
depth of the stack in micrometers.
The
Slices
input box displays the number of slices for the stack. This number can be edited by typing or
using the arrow keys. Changing the value for
Slices
automatically adjusts the
Interval
in a way that the
total
Range
for the stack is maintained.
The
Interval
input box displays the z-distance (interval) between slices in
µm
. This number can be edited
by typing or using the arrow keys. Changing the value for the
Interval
automatically adjusts the
Number
of Slices
while keeping the total
Range
for the stack within the possibilities of fitting the calculated
number of slices within the Range. The total Range may therefore vary with higher and lower numbers
around the
initial value of the Range.
Fig. 94
Z-Stack Navigation appearance,
performing a Z-Stack using
First/Last Mode
Summary of Contents for Lightsheet Z.1
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Page 427: ...Lightsheet Z 1 Overview ...
