ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
189
5.3.14.3
Localization Microscopy Tools –
Localization Precision
Select
Localization Precision
to attach a
Localization Precision look up table (LUT) to an
Experimental PSF (Fig. 299).
Highlight the Image with the Experimental PSF
(should be derived from the PSFs obtained by a 4
µm stack of 100 nm beads) and press
Select
(Fig. 300).
Press apply to obtain a file of the Experimental PSF
with LUT attached. The file bears the extension
"LocalizationPrecision".
Please note that the localization precision
will be estimated using a Monte-Carlo
Simulation of the experimental PSF including
noise. The generated lookup table will then
contain the localization precision depending
on the three parameters photon number,
background variance and Z position.
The Experimental PSF with attache LUT
should be used when fitting 3D SMLM data.
If only an Experimental PSF without LUT is
used, a warning message pops up (Fig. 301).
Press
No
to cancel processing or
Yes
if you
want to continue. In this case, only a rough
estimate of the localiszation precision will be
given.
Fig. 299
Processing – Localization Precision tool
selected
Fig. 300
Processing – SMLM - Localization
Precision with double-lobed
Experimental PSF loaded
Fig. 301
Localization precision warning