INSTRUMENT DESCRIPTION
Carl Zeiss
Diaphragm Size and Objective Magnification
DirectFRAP
30
423635-9001-701
05/2014
2.5
Diaphragm Size and Objective Magnification
Diaphragm Edge
Objective Magnification and Diaphragm Size (µm)*
DirectFRAP without TIRF Slider
DirectFRAP with TIRF Slider
Clockwise
Operation
Position
Type**
40x
63x
100x
40x
63x
100x
1
3.6
2.3
1.5
3.0
1.9
1.2
2
7.5
4.8
3.0
6.2
4.0
2.5
3
11.2
7.1
4.5
9.3
5.9
3.7
4
15.1
9.6
6.0
12.5
8.0
5.0
5
19.0
12.1
7.6
15.8
10.0
6.3
6
3.6
2.3
1.5
3.0
1.9
1.2
7
7.5
4.8
3.0
6.2
4.0
2.5
8
11.2
7.1
4.5
9.3
5.9
3.7
9
15.1
9.6
6.0
12.5
8.0
5.0
10
20.1
12.8
8.0
16.7
10.6
6.6
11
6.1 / 20.1
3.9 / 12.8
2.5 / 8.0
5.1 / 16.7
3.2 / 10.6
2.1 / 6.6
12
Free position***
13
5.0 / 20.1
3.2 / 12.8
2.0 / 8.0
4.2 / 16.7
2.7 / 10.6
1.7 / 6.6
14
10.2 / 20.1
6.4 / 12.8
4.0 / 8.0
8.4 / 16.7
5.3 / 10.6
3.3 / 6.6
15
5.0 / 20.1
3.2 / 12.8
2.0 / 8.0
4.2 / 16.7
2.7 / 10.6
1.7 / 6.6
16
10.1 / 20.1
6.4 / 12.8
4.0 / 8.0
8.4 / 16.7
5.3 / 10.6
3.3 / 6.6
17
5.0 / 20.1
3.2 / 12.8
2.0 / 8.0
4.2 / 16.7
2.7 / 10.6
1.7 / 6.6
18
10.1 / 20.1
6.4 / 12.8
4.0 / 8.0
8.4 / 16.7
5.3 / 10.6
3.3 / 6.6
19
5.0 / 20.1
3.2 / 12.8
2.0 / 8.0
4.2 / 16.7
2.7 / 10.6
1.7 / 6.6
20
10.1 / 20.1
6.4 / 12.8
4.0 / 8.0
8.4 / 16.7
5.3 / 10.6
3.3 / 6.6
*
The diaphragm sizes indicated refer to the diaphragm image in the specimen plane; the measures indicate diameters and
edge lengths, respectively.
**
The orientation of the rectangular diaphragms is shown as required for the AxioCam standard position on the left
sideport.
***
Edge sharpness focusing is not possible.
Summary of Contents for DirectFRAP
Page 1: ...DirectFRAP Operating Manual...
Page 62: ......
