3D Microscopy
Axiovert 100/135/135 M
Eyepiece version
B 40-022-4 e 09/98
21
2.3
Adjustment
Fig. 7
Adjustment of 3D illumination shutter,
pupil
Use cover slip as an object and focus on it
using the 10x objective;
after adjustment, check the setting using the
objective of the highest magnification
2.3.1 Adjustment of 3D illumination
shutter
Move rotary switch of the 3D-CONTROL
DSA-3 to the
STEP
position:
Fig. 6
Observe the pupil:
Axiovert 100: remove the eyepiece and
insert the centering telescope,
Axiovert 135/135 M with Bertrand lens in
the 4x turret:
use centering telescope or Bertrand lens to
focus on the aperture diaphragm;
one pupil half will be bright, the other dark:
Fig. 7
Move 3D illumination shutter to the side
until both pupil halves appear identical in
size;
if required, reset the stop screw;
Use fixation screw to hold 3D illumination
shutter in position
Close aperture diaphragm;
check center position
