OPERATION
Axioskop
2
plus
Carl Zeiss
Illumination and contrasting techniques
Axioskop 2
mot plus
3-32
B 40-075 e 02/01
3.3.4.2
Determination of the vibration direction n
γ
'
(1) Application
The determination of vibration directions n
γ
, n
γ
' (direction with the absolutely and relatively highest
refractive index), n
α
and n
α
' (direction with the absolutely and relatively lowest refractive index) in
relation to morphological directions, e.g. of crystal surfaces, crystal needles or fibers, is an important
criterion for recognition. It is also used for the diagnosis of biocrystals (e.g. gout, pseudogout).
Fig. 3-23
Determination of the vibration direction n
γ
'
using the example of an artificial fiber
(2) Axioskop
2
plus
and Axioskop 2
mot plus
configuration
−
Eyepiece with reticle
−
Objectives suitable for polarization
−
Rotary mechanical stage 75x50/240° R (3-22/
)
−
SENARMONT DIC polarizer or polarizer D (rotary or fixed), unless a condenser with integrated polarizer
is used.
−
Analyzer module D in reflector turret
−
Adjusting specimen for polarization microscopy (453679-0000-000)
(3)
Setting of Axioskop 2
plus
and Axioskop 2
mot plus
•
Set the microscope as for transmitted-light brightfield (see section 3.3.1 (3)), taking care to ensure
that the interpupillary distance of the binocular tube has been correctly set (see section 2.1.3 (2)).
•
Center the rotary mechanical stage (3-22/
) (see section 2.2.9 (2)).
•
) in beam path and position it to 0°, provided that a rotary polarizer is used.
•
Swing in analyzer module on reflector turret (3-22/
). The field of view now appears dark on account
of the crossed polarizers.
•
Place Pol adjusting specimen on the microscope stage and turn the stage until the adjusting specimen
is in dark position.
•
Switch off analyzer and align crosslines with the cracks of the object.
•
Then switch on analyzer again and remove the adjusting specimen. The transmission directions of
polarizer and analyzer are now parallel to the crosslines (polarizer E-W, analyzer N-S).
