ZEISS
5 Operation | 5.4 Setting Up for Transmitted Light Techniques
5.4.8 Setting Up for Transmitted Light Polarization
The following requirements must be fulfilled:
§
The microscope is operational.
§
Strain-free objectives are installed in the
§
§
A condenser with polarizer or the D Polarizer is
§
The analyzer module Pol ACR P&C for transmitted light in the reflector turret/slider or the ana-
lyzer slider D fixed or with lambda plate is installed.
§
A depolarizer for avoiding unwanted polarizing effects is installed.
§
The microscope is adjusted for
transmitted light brightfield microscopy [
.
§
§
The Pol objectives are
.
5.4.8.1 Detecting Birefringence
For more information about the method, see chapter
.
2
1
3
1
Rotary stage
2
Polarizer
3
Analyzer
Procedure
1. Swing the polarizer
2
into the beam path.
2. When using a rotatable polarizer, position it to 0°.
3. Put the analyzer
3
into the slit for the compensator.
à
The field of view appears dark.
4. Bring the sample into the field of view.
5. With the rotary stage
1
, turn the sample.
à
Normally, birefringent (anisotropic) objects will now show the interference color and in-
tensity variations during rotation between crossed polarizers.
Optically anisotropic substances may remain dark when an isotropic direction, e.g. from
optically single-axle or double-axle crystals, is oriented parallel to the observation direc-
tion.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001
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