OPERATION
ZEISS
Illumination and contrast techniques
Axio Observer
146
431004-7244-001
12/2016
5.12.1.2
Transmitted light brightfield instrument equipment
Every Axio Observer microscope is equipped with the components required to carry out transmitted light
bright field microscopy.
5.12.1.3
Setting transmitted light brightfield according to KÖHLER (example: stand 5)
−
The microscope has been started properly as described in section
−
The microscope has been switched on.
•
Select the objective with the lowest magnification (e.g. 10x - yellow code ring) on the nosepiece
(Fig. 148/
23
), ensure that it clicks into position correctly.
•
Set factor 1x on the selector wheel (Fig. 148/
27
) of the Optovar turret (selector wheel only available on
5 and 5 materials stands); ensure that it clicks into position correctly.
•
Open the luminous-field diaphragm completely by turning the luminous-field diaphragm control
(Fig. 148/
10
) on the carrier for transmitted-light illumination to the left.
•
Open the aperture diaphragm completely by rotating the selector wheel on the condenser forwards as
far as it will go.
•
Turn the condenser turret selector wheel (Fig. 148/
15
) to move the condenser turret to the
H
position
for brightfield (if H is not available, to the
DIC
position).
•
Turn the adjustment ring to move the reflector turret (if available) to a position which contains no filter
combination. Ensure that it clicks into position correctly.
•
If necessary remove the analyzer slider from the slot or move to the open position. Ensure that it clicks
into position correctly.
•
Rotate the sideport control wheel (Fig. 148/
5
) to the 100 % vis (visual) position (only for 3 and 5
stands, motorized for 7 stands).
•
Set the beam splitting ratio to 100 % vis on the binocular (photo) tube. Remove the Bertrand lens
from the optical path (where used). To do this, move the rotary / sliding button to the 100 % vis
position.
•
Swivel the 3-position filter changer (Fig. 148/
9
) out of the optical path.
•
Place a high-contrast specimen on the microscope stage (Fig. 148/
7
).
•
Match the eyepiece distance (interpupillary distance) to the user's individual interpupillary distance: For
this purpose, pull apart or push together the binocular component (Fig. 148/
35
) of the tube.
•
Set the ametropia correction zero point using the adjustment ring (Fig. 148/
37
) of the eyepieces
36
):
without eyepiece reticles:
set to the white point,
with eyepiece reticles:
set to the red point,
•
To correct for ametropia, bring the selected detail of the specimen into optimum focus using the
relevant eyepiece adjustment ring.
•
Use the coarse / fine focus drive (Fig. 148/
28
) to focus on the selected detail of the specimen. If no
light is visible in the eyepieces, check that light is being emitted from the housing of the halogen
illuminator. If no light is being emitted, switch on the halogen illuminator by pressing the TL button
(Fig. 148/
30
).
Summary of Contents for Axio Observer Series
Page 1: ...Axio Observer Inverted microscope Operating manual...
Page 192: ......