Leica StereoZoom User Manual Download

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Leica

StereoZoom

User Manual

Summary of Contents for StereoZoom

Page 1: ...Leica StereoZoom User Manual ...

Page 2: ... 17 Leica L2 cold light source 17 Graticules 18 Camera assembly for Leica S6 D and S8 APO 20 Operating Controls 22 Interpupillary distance and exit pupil 23 Eyecups 24 Setting working distance and focusing 24 Zoom magnification changer 25 Diopter adjustment 28 Photography with Leica S6 D and S8 APO 32 Special notes What do I do if 33 Care and maintenance 34 Calculating total magnification and fiel...

Page 3: ...ols to quality assurance functions The stereomicroscopes cold light source and stand are all antistatic to protect against ESD A Terminator version is available for problematic working areas in electronics One of our major development goals was to make our new StereoZoom line simple and intuitive to use Never theless please take the time to read these instructions They will familiarize you with yo...

Page 4: ... regulations laws and directives The Leica S series can be used in clean rooms without any problems and they can be cleaned as described on page 35 Please observe the following rules Do not clean our stereomicroscopes and accessories in a different manner from that described in this user manual Do not use unsuitable cleaning agents chemicals and techniques Do not clean painted surfaces and accesso...

Page 5: ...l risk of infection The risk can be kept at a minimum by using individual eyepieces or attachable eyecups see p 17 Electrically operated equipment Handle liquids carefully Liquids spilt on the instrument can cause the stereomicroscope and other equipment to become electrically live and injure persons can cause damage to the instrument Check regularly to ensure that the mains cable is undamaged and...

Page 6: ...the mains Wait for filament lamps to cool down before changing Touching hot filament lamps can result in burns Observe all statutory accident prevention and environmental protection requirements National laws and regulations must be observed for the dispo sal of these products Electrically operated accessories for Leica S4 E S6 E S6 S6 T S6 D and S8 APO stereomicroscopes are manufactured using sta...

Page 7: ... our homepage at www leica microsystems com Symbols in this manual Safety warnings This symbol denotes information that must be read and acted upon without fail Disregarding safety warnings can put persons at risk Disregarding safety warnings can also result in malfunctioning or damage to the instrument Warning against exposed hot points e g filament lamps Risk of burns if disregarded Important in...

Page 8: ...ptical performance with large flat distortion free fields of vision and high contrast images with maximum chromatic correction The Leica S4 E S6 E S6 S6 D and S8 APO stereomicro scopes including cold light source and stand are made of antistatic material with a surface resistance of 2 1011 ohm square and a discharge time from 1000V to 100V of under 2 seconds The Leica S6 T Terminator for problemat...

Page 9: ...gistered as a trademark in the Principal Register of the US Patent and Trademark Office World first Leica StereoZoom S8 APO is the first stereomicroscope on the market with completely apochromatic corrected Greenough system Apochromatic optics corrects per fectly chromatic aberrations eliminates interfering color fringes and renders even the finest details ultra sharp Contrast brilliance sharpness...

Page 10: ...10 Leica StereoZoom User Manual Description StereoZoom Leica S4 E StereoZoom Leica S6 E StereoZoom Leica S6 Leica Design by Christophe Apothéloz ...

Page 11: ...11 Leica StereoZoom User Manual Description StereoZoom Leica S6 T StereoZoom Leica S6 D StereoZoom Leica S8 APO ...

Page 12: ...pieces Eyepieces fixed Eyepieces adjustable Standard ergonomic Standard ergonomic 10 23 10 447 130 10 23 10 447 131 16 16 10 446 354 10 447 132 16 16 10 446 355 10 447 133 20 12 10 446 356 10 447 134 20 12 10 446 357 10 447 135 Adjustable eyepieces accept reticles 10 447 057 10 447 065 10 447 058 10 447 153 Leica S4 E 10 446 293 7 5 6 3 1 1 StereoZoom Optics carriers 2 Objectives 3 Eyepieces 4 Car...

Page 13: ...47 139 10 447 036 25 9 5B 10 445 302 Spacing Ring required with eyepieces 40 6B 10 445 303 10 445 302 10 445 303 Photo Video documentation Leica DC cameras Objectives for S4 E S6 E S6 S6 T S6 D 0 32 10 446 316 0 5 10 446 318 0 63 10 446 319 0 75 10 446 320 1 6 10 446 321 2 10 446 322 Lens 10 446 324 shield Adjustable Lens 0 3 0 4 10 446 325 Ergolenses 0 6 0 75 10 446 323 0 7 1 10 446 317 Objective...

Page 14: ...d light base with glass stage plate 3 Focus column with microscope carrier 4 StereoZoom optics carrier 5 Optional additional objective 6 Eyepieces fixed and or adjustable The components 14 Leica StereoZoom User Manual Design Basic equipment 5 4 3 2 1 6 ...

Page 15: ... 왘 Remove glass stage plate 왘 Undo locking device 왘 Place incident light stand on the transmitted light base and engage in the connecting screw 왘 Close locking device 왘 Replace glass stage plate 왘 Insert universal light guide in the socket at the back see user manual for the Leica L2 cold light source Focus column Incident light base Transmitted light base Incident light base 15 Leica StereoZoom U...

Page 16: ...s it will go 왘 Check that it sits firmly and snugly You can combine your StereoZoom with a fixed and an adjustable eyepiece Two adjustable eyepieces are re quired for equipment which has a reticle in an eyepiece for measurement or photography purposes We recom mend that you also equip the high performance Leica StereoZoom S8 APO with two adjustable eyepieces Optics carrier Stand Additional objecti...

Page 17: ...microscopes Mat ching adapters are available for connecting the Leica L2 cold light source to various stereomicroscope stands and for standalone operation Detailed information on the construction and use of the Leica L2 can be found in the relevant user manual Please note that the universal light guide on the Leica S8 APO can only be used with the sideways mounted lamp arm For higher requirements ...

Page 18: ...ilable Two adjustable eyepieces are required for equipment which has a reticle in an eyepiece for measurement or photography purposes The graticules can be inserted into adjustable eyepieces and eyepieces for spectacle wearers 왘 Ascertain with the aid of the stereomicroscope on which side the vacuum metalized scale is located The scale should be visible on the correct side 왘 Pull out the insert fr...

Page 19: ...fingerprints and slide into the holder from the side 왘 Put the insert back into the eyepiece and press firmly in place 왘 Insert the eyepiece into the eyepiece tube and line up the graticule by turning the eyepiece in the eyepiece tube The measuring process is described in the Measuring instructions ...

Page 20: ...usings 10 445 929 1 2 0 5 10 445 928 1 3 0 32 11 541 006 1 1 11 541 007 2 3 0 63 11 541 016 1 2 0 5 1 2 3 1 2 1 3 C Mount CCD cameras Leica DC cameras 1 C Mount CCD camera 3 Chip 1 2 B Mount CCD camera 3 Chip 2 3 B Mount CCD camera 11 543 702 10 445 930 1 10 446 175 10 446 261 2 3 0 63 10 446 307 2 3 0 8 Leica S6 D 10 446 297 Leica S8 APO 10 446 298 Assembly Leica StereoZoom User Manual ...

Page 21: ...the dust cover if there is no camera attached 왘 Attach appropriate video or photo objective accor ding to which camera is used see scheme p 20 The format reticle is used to test the sharpness and picture detail Borderlines are displayed on the reticle for all film formats The left observation beam path and the photo output are aligned with one another Always judge therefore the picture detail and ...

Page 22: ...stable eyepiece tubes Interpupillary distance adjustable from 55 75mm 6 Eyepieces and eyecups 7 Lamp bracket mounting thread on both sides and at rear 8 Socket for connecting for the Leica L2 universal light guide not for Leica S8 APO 9 Thread for additional objective lens shield Overview Standard controls and their functions Leica StereoZoom User Manual Operating Operating 6 5 2 4 1 9 7 3 7 8 ...

Page 23: ... distance of 77 137 mm and the 0 7x 1x ErgoLens with variable working distance of 48 mm 98 mm permit fine adjust ment of magnification working distance as well as viewing height without time consuming lens changes Leica S6 with 60 viewing angle offers optimal comfort at the tilted stereomicroscope The interpupillary distance can be adjusted from 55 75mm The exit pupil is the distance between the e...

Page 24: ...il the desired object segment is in focus i e inside the objective s working distance The working distances of the various objectives are listed in the tables on pages 38 40 You can operate the focusing drive with either your right or left hand 왘 Position the object under the objective 왘 Select the minimum magnification You should select the minimum magnification because it is easier to find the d...

Page 25: ...t All StereoZoom models are equipped with a continuously adjustable magnification changer that can be operated with either the right or left hand The right hand pinion knob carries a magnification scale The ranges are 0 63 to 3 on the S4 E and 0 63 to 4 on the S6 models for S8 APO from 1 to 8 The tables on pages 38 to 40 list the magnifications and field diameters as a function of magnification ch...

Page 26: ...Set the minimum zoom level at 1 using the stop on the left hand pinion knob 왘 Loosen the hexagon head screws on the left hand pinion knob using the Allen key supplied 왘 Turn the right hand pinion knob to 1 왘 Move the stop on the left hand pinion knob forwards until it touches the built in zoom stop 왘 Tighten the hexagon head screws Setting the zoom limiter Example Setting a zoom range of 1 to 3 2 ...

Page 27: ...sing the stop on the right hand pinion knob 왘 Loosen the hexagon head screws on the right hand pinion knob 왘 Turn the right hand pinion knob to 3 2 왘 Move the stop on the right hand pinion knob backwards until it touches the built in zoom stop 왘 Tighten the hexagon head screws 3 2 ...

Page 28: ...owing adjustments only once If you are using a graticule the diopter adjustment and parfocality setting procedure is slightly different to that described here Please refer to the section on measure ment in the graticule user manuals Setting up diopter with an adjustable and a fixed eyepiece 왘 Set up illuminator 왘 Set interpupillary distance p 23 왘 Set approximate working distance using the focusin...

Page 29: ...e test object and slowly turn the eyelens clock wise the direction until the object is in focus 왘 Set the microscope to maximum magnification 왘 View the test object with both eyes and bring it into sharp focus with the focusing drive View the object while zooming from minimum to maximum magni fication The object should remain in constant focus parfocal at all times If it does not please repeat the...

Page 30: ...ocusing drive working distances of various objectives see p 38 40 왘 Set up lighting 왘 Set viewing distance p 23 왘 Set 0 diopters on both eyepieces 왘 Place flat test object under the objective 왘 Set lowest magnification 왘 View test object through the eyepiece and focus using the focusing drive 왘 Set highest magnification 왘 Optimize sharpness using the focusing drive Preparations Focusing on test ob...

Page 31: ... turn the lens slowly clockwise in the direction until this eye sees the object clearly 왘 Set the diopter for the other eye in the same way 왘 Select highest magnification 왘 View object and if necessary gently refocus 왘 Adjust magnification changer from the lowest to the highest magnification When doing this the sharp ness must remain constant over the entire zoom range parfocal Otherwise please re...

Page 32: ...piece no light in the left eyepiece 100 light in the video photo beam path Focusing and framing down the left eyepiece video photo beam path 왘 When the picture detail and sharpness are set to your satisfaction switch to the Doc position and take your photo Please note Images of flat specimen are partly unsharp left and right edge Leica StereoZoom User Manual DOC VIS Operating Photography with Leic...

Page 33: ...he mains power switch is in the on position that the mains power cable is correctly connected that all connector cables are correctly connected that none of the fuses have blown the image is too dark Set regulating knob high enough Photographs are blurred Focus precisely p 24 Focus on reticles and carry out diopters correction exactly according to the instructions p 30 Insert eyepieces all the way...

Page 34: ... user manual gives express instructions for doing so against oil and grease Never grease guides and mechanical parts Cover your microscope with its dust cover during breaks Put dust covers over tube openings eyepieces and eyepiece tubes without eyepieces Remove dust with a rubber blower and soft brush Clean eyepieces and objectives with special optical cleaning cloths and pure alcohol Keep accesso...

Page 35: ...c may become brittle and eventually break with caustic or acetone containing substances such as ether substitute with any other solvents except ethanol and isopropanol warm soapy water rinsing the plastic afterwards with distilled water ethanol industrial alcohol and isopropanol Always take appropriate safety precautions when using ethanol and isopropanol Cleaning plastic parts Never clean plastic...

Page 36: ...om negligence or incorrect handling If you treat your valuable optical instrument with due care it will repay you with the decades of reliable precision for which Leica instruments are renowned However should you encounter problems with your micro scope at any time please contact your local Leica represen tative or Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg We guarantee quality Special n...

Page 37: ... number Field numbers are printed on the eyepieces 10 23 16 16 20 12 10 23B 16 14B 25 9 5B 40 6B Example MO 1 6 objective ME 20 12 eyepiece z Zoom position 4 0 Magnification in the binocular tube MTOT VIS MO ME z 1 6 20 4 128 Field diameter in the object NFOV 12 OF __________ __________ 1 9mm MO z 1 6 4 Calculating total magnification and field diameter ...

Page 38: ... 2 31 5 40 3 50 4 6 4 8 1 10 1 12 6 16 1 20 2 25 2 32 3 40 3 9 9 12 6 15 8 19 7 25 2 31 5 39 4 50 4 63 0 15 9 20 2 25 2 31 5 40 3 50 4 63 0 80 6 100 8 57 5 46 0 36 5 29 1 22 8 18 3 14 6 11 4 9 1 40 0 31 6 25 3 20 3 15 9 12 7 10 2 7 9 6 4 30 4 23 8 19 0 15 2 11 9 9 5 7 6 6 0 4 8 35 0 27 7 22 2 17 8 13 9 11 1 8 9 6 9 5 6 24 0 18 8 15 0 12 1 9 4 7 5 6 0 4 7 3 8 15 1 11 9 9 5 7 6 6 0 4 8 3 8 3 0 2 4 4...

Page 39: ... 3 10 7 8 5 6 7 5 3 25 3 20 0 16 0 12 8 10 0 8 0 6 4 5 0 4 0 29 5 23 3 18 7 14 9 11 7 9 3 7 5 5 8 4 7 20 1 15 8 12 6 10 1 7 9 6 3 5 1 4 0 3 2 12 7 10 0 8 0 6 4 5 0 4 0 3 2 2 5 2 0 3 5 4 5 5 6 7 0 9 0 11 2 14 0 17 9 22 4 5 6 7 2 9 0 11 2 14 3 17 9 22 4 28 7 35 8 7 1 9 0 11 2 14 0 17 9 22 4 28 0 35 8 44 8 5 6 7 2 9 0 11 2 14 3 17 9 22 4 28 7 35 8 8 8 11 2 14 0 17 5 22 4 28 0 35 0 44 8 56 0 14 1 17 9...

Page 40: ...2 4 128 0 160 0 201 6 256 0 50 0 62 5 80 0 100 0 125 0 160 0 200 0 250 0 315 0 400 0 80 0 100 0 128 0 160 0 200 0 256 0 320 0 400 0 504 0 640 0 11 5 9 2 7 2 5 8 4 6 3 6 2 9 2 3 1 8 1 4 8 0 6 4 5 0 4 0 3 2 2 5 2 0 1 6 1 3 1 0 4 8 3 8 3 0 2 4 1 9 1 5 1 2 1 0 0 8 0 6 3 0 2 4 1 9 1 5 1 2 0 9 0 8 0 6 0 5 0 4 3 2 4 0 5 1 6 4 8 0 10 2 12 8 16 0 20 2 25 6 5 1 6 4 8 2 10 2 12 8 16 4 20 5 25 6 32 3 41 0 8 0...

Page 41: ...ereoZoom User Manual 41 with incident light stand Dimensions Leica S4 E S6 E S6 T 161 107 23 25 81 110 351 3 8 22 134 223 278 393 ø58 ø76 46 278 393 205 144 104 113 148 205 Leica S4 E S6 E S6 T Dimensions in mm ...

Page 42: ...5 81 110 351 3 8 22 65 134 223 278 393 ø58 ø76 46 with transmitted light stand Dimensions Leica S4 E S6 E S6 T 144 104 113 148 205 220 278 393 205 Leica S4 E S6 E S6 T Dimensions in mm 42 Leica StereoZoom User Manual ...

Page 43: ...5 144 104 113 148 205 43 with inclining focusing drive Dimensions Leica S6 Leica StereoZoom User Manual Dimensions Leica S6 D 120 148 25 81 110 390 6 0 ø15 8 25 26 35 ø58 ø76 46 144 104 150 4 Leica S6 Dimensions in mm Dimensions in mm Leica S6 D with incident light stand ...

Page 44: ... Leica StereoZoom User Manual 207 107 23 25 81 110 351 3 8 22 65 134 223 278 393 ø58 ø76 171 278 393 205 144 104 113 148 205 220 Leica S6 D with incident light stand Dimensions Leica S6 D Dimensions in mm ...

Page 45: ...m User Manual 45 Leica StereoZoom User Manual with incident light stand Dimensions Leica S8 APO 207 107 23 25 116 75 351 3 8 22 134 223 278 393 ø58 ø76 171 278 393 205 144 104 113 148 205 Dimensions in mm Leica S8 APO ...

Page 46: ...h transmitted light stand Dimensions Leica S8 APO 207 107 23 25 116 75 351 3 8 22 65 134 223 278 393 ø58 ø76 171 144 104 113 148 205 220 278 393 205 Dimensions in mm Leica S8 APO Leica StereoZoom User Manual ...

Page 47: ...andards are in force in each of the Group s 11 pro duction sites in seven countries making the name Leica synonymous with quality Technology is one key factor in the success of Leica Microsystems customer focus the other Our friendly staff offer competent on the spot advice and service in your own language Naturally we also cater reliably and without fuss for all customer wishes and ensure that yo...

Page 48: ... The companies of the Leica Microsystems Group operate internationally in four business segments where we rank with the market leaders Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visualization measurement and analysis of microstructures in life sciences and industry With confocal laser technology and image analysis systems we provide three dimensional view...

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