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OPERATION
Carl Zeiss
Illumination and contrast methods
Axio Imager
172
430000-7344-001
M70-2-0020 e 06/2009
•
Place the specimen on the stage, focus on it
and rotate the control wheel (4-129
) on the
C-DIC slider
6x20 or the modulator turret
1
) until the specimen detail of interest is
visible at maximum contrast. Rotating the stage
is no longer necessary. If both line marks are
facing each other (4-130/
3
), this corresponds to
the mid-position. Rotation by 45° in both
directions is possible).
•
The contrast can be optimized by turning
setscrew (4-129/
2
) on the C-DIC slider or on the
modulator turret (4-130/
2
). If triangular and line
mark are facing each other (4-130/
3
), this
corresponds approximately to the extinction
position (best contrast).
(5) Motorized four-position modulator
turret for circular DIC/TIC
•
Set the microscope up for reflected-light
brightfield.
•
Put reflector module C DIC P&C in the light
path.
•
On the nosepiece, swivel in the objective
suitable for DIC.
•
On the modulator turret, swivel in the
appropriate DIC prism by
pressing
on knob
•
1
) for forward rotation or on knob
2
) for backward rotation of the turret.
•
Put on the specimen, focus and turn knob
(4-131/
2
) (
SHIFT
) in such a way that the
structure of interest can be seen in maximum
contrast.
•
Contrast can be optimized by turning knob
(4-131/
1
) (
AZIMUT
) on the modulator turret.
Fig. 4-130 4-position modulator turret
Fig. 4-131 Motorized four-position modulator
turret for circular DIC/TIC