OPERATION
Carl Zeiss
Illumination and contrast methods
Axio Imager
152
430000-7344-001
M70-2-0020 e 06/2009
•
If congruence is not perfect (4-119/
A
), use the
two SW 1.5 screwdrivers (4-118/
1
and
4
) for
turning the two centering screws (4-118/
2
and
3
) to recenter the bright phase stop until
complete congruence with the dark phase ring
is achieved (4-119/
B
).
•
Finally, remove the auxiliary microscope from
the tube and reinsert the eyepiece.
Normally, however, centering is not required, since
the phase stops are factory-centered and centering
is retained even if the universal condenser is
removed from the condenser carrier and attached
again.
To enhance the image contrast, an interference
wide-band filter, green 32 x 4, can be inserted in
the color glass holder (requires color glass holder
for 32 mm filter size).
Perfect phase contrast is only achieved if the bright
phase stop (in the condenser) and the dark phase
ring (in the objective) are exactly congruent in the
illumination beam path (4-119/
B
).
Fig. 4-118 Centering the phase stop on the
universal condenser
Fig. 4-119 Centering the phase stop (bright
ring in condenser) to the phase ring
(dark ring in objective)
