Axio Imager 2
FIRST-TIME SET-UP
ZEISS
Rotary stage Pol
01/2016
430000-7544-001
75
3.36.2
Attaching rotary stage Pol
•
Where necessary, loosen screw cap (Fig. 67/
2
) of the spring housing with about three rotations.
•
Place rotary stage Pol (Fig. 67/
5
) with the groove of the dovetail (stage bottom) on spring-loaded pin
3
).
•
Attach the rotary stage with the clamping screw (Fig. 67/
4
) pointing to the front right.
•
Press the rotary stage Pol to the front against the spring-loaded pin and lower it towards the back into
the stage carrier (Fig. 67/
1
), then release it.
•
Retighten screw cap (Fig. 67/
2
).
3.36.3
Centering rotary stage Pol
With high-power objectives, stage centricity can
only be exact for one selected objective.
The centricity will be exact for all objectives, if an
objective nosepiece Pol is used.
In this case, turn to the objective position with DIC
slot.
All stages are factory-precentered, i.e. when the
stage is rotated, the set specimen detail will remain
in the center of the image. If the detail moves out
of the center of the field of view (Fig. 68/
5
) while
rotating the stage, the stage should be recentered
by following this procedure:
•
Before centering the stage, the microscope
illumination must first be adjusted using
KÖHLER's rules (see Section 4.12.1).
•
To center the stage, use a high-contrast
specimen and an eyepiece with crossline reticle.
•
Loosen the stage ratchet (Fig. 68/
4
) or stage
clamp (on rotary stage Pol with clamping
device) and the screw cap of the stage carrier
(Fig. 68/
1
).
•
Rotate the stage to determine the maximum
specimen deflection (Fig. 68/
5
, tip of arrow) to
the eyepiece reticles.
•
Turn each of the two centering screws on the
stage carrier (Fig. 68/
2
) using an SW 1.5 Allen
key (Fig. 68/
3
) to move the specimen detail by
half of the arrow length towards the reticle
center. Check whether the specimen detail
moves when the stage is rotated again; repeat
the procedure, if required.
•
When centering is finished, tighten screw cap
(Fig. 68/
1
) again.
Fig. 68
Centering rotary stage Pol