Zeiss Axio Examiner, Operating Manual, Page: 68 / 96

OPERATION
Carl Zeiss Illumination and contrast procedures Axio Examiner
68 M60-2-0003 e 05/2012
(3) Switch to reflected-light fluorescence
The first reflected-light fluorescence setting will be
considerably easier if you begin with a highly
fluorescent specimen. But demonstration
specimens may also be used at the beginning.
•
Switch on the HAL100 halogen lamp.
•
Switch the condenser turret first to the
H
position for a transmitted-light bright field, and
go the specimen position to be examined.
•
Keep the optical path in the reflected-light
illuminator blocked using the blocking position
of the reflected-light filter drawer (Figure 4-8/
2
)
for the time being.
•
Switch on the HBO 100 or HBO 50
illuminator (Figure 4-8/
1 ) at the illuminator's
transformer (see section 3.2.3), and allow
approx. 15 min to heat up to operating
temperature.
•
Select and switch on the FL P&C reflector
module with the desired fluorescence filter
combination (depending on the type of
excitation) at the reflector turret (Figure 4-8/
6 ).
•
Enable the optical path in the reflected-light
illuminator when using the reflected-light filter
drawer (Figure 4-8/
2 ).
•
If required, set the illumination intensity of the fluorescent illuminator (HXP/HBO) using the thumb
wheel at the FL attenuator (Figure 4-8/
3
).
•
Close the luminous-field diaphragm (Figure 4-8/ 4 ) until it becomes visible in the visual field.
•
Turn the two centering screws (Figure 4-8/ 5 ) to center the luminous-field diaphragm to the visual field
border.
•
Either open the luminous-field diaphragm until it just disappears behind the visual field border, or
reduce the luminous-field diaphragm up and into the visual field if there is a risk that the specimen
fades out.
•
Finally, refocus to the specimen, and optimize the HBO 100 100 / HBO 50 collector position if
required. Set the collector to a value at which the visual field illumination appears as uniform as
possible using the shortwave excitation reflector module. No correction of the collector position is
required any longer for modules with excitation at a longer wavelength.
Figure 4-8 Components for reflected-light
fluorescence (Axio Examiner.A1)