YSI 1500 Sport, User Manual, Page: 47 / 134

6 6 6 6----1 1 1 1
6. Instrument Operation
IMPORTANT IMPORTANT IMPORTANT IMPORTANT: You should check the probe current before beginning tests. (See
section 7 Instrument Diagnostics. If there are any problems, you don't want to
discover them after you have begun.
Step by step instructions in this chapter will help you learn the operation of the
SPORT. Instructions for each step are followed by an illustration of the display
as it should respond. If the display does not respond correctly, press MENU
MENU MENU MENU and
try again. It is not uncommon for an instrument to be unstable for the first hour
or so once it has been initially setup and calibrated. This is a result of the
membrane requiring time to break in and also because the surface of the enzyme
probe requires time to condition itself with respect to the new membrane and
fluids. As a result of this YSI recommends that in the first hour of use after
As a result of this YSI recommends that in the first hour of use after As a result of this YSI recommends that in the first hour of use after
As a result of this YSI recommends that in the first hour of use after
initial setup the instrument should be checked several times for accuracy initial setup the instrument should be checked several times for accuracy initial setup the instrument should be checked several times for accuracy initial setup the instrument should be checked several times for accuracy
(use YSI standards), and calibrated as often as required.
(use YSI standards), and calibrated as often as required. (use YSI standards), and calibrated as often as required. (use YSI standards), and calibrated as often as required.
6.1 Calibration Cycle
There are two modes of operation allowed on the SPORT, Automatic and
Manual mode. Each have their own calibration process which are described in
the sections that follow.
Calibration Calibration Calibration Calibration----Automatic Mode Automatic Mode Automatic Mode Automatic Mode
In Automatic mode, you should calibrate the SPORT on these occasions:
•
Every day at the beginning of testing
•
Whenever you change the membrane or install new reagent fluids
•
Whenever you turn the instrument on (automatically prompted)
•
Whenever you change injection devices
It is recommended that a sample of the 5 mmol calibration standard should be
ran at least once every two hours while testing in order to check calibration
status. If the result is outside the specified range, then recalibration is necessary.
Prepare buffer and reference solutions and have the 5 mmol/L, 15 mmol/L and
30 mmol/L calibration standards provided in the 1504 Starter Kit at hand. With
the SPORT set up as described in Section 3.3, proceed as follows:
Load the Syringepet or injector with 5 mmol/L standard.
WARNING: WARNING: WARNING: WARNING: If you calibrate with the Syringepet, measure measure measure measure with the Syringepet.
If you calibrate with the injector, measure
measure measure
measure with the injector. Otherwise, you
may get incorrect values.