YL Instruments YL9100 HPLC, User Manual

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YL9110 Quaternary Pump
2-5. Gradient curve
Y
L9110
Solvent A and solvent B are mixed with flow rate Fb,
Mixed solvent flows to the column with flow rate Fab,
Solvent volume V of solvent A vs Concentration of solvent B
C(%) = 100 x {1 - (F
ab
-F
b
)/V x t}
(Fb/Fab-Fb)
The solvent ratio difference between present row and next row increases or decreases as a exponential
curve. In general, the gradient elution is used for separation of duplicated peaks. YL9110 quaternary
pump has a exponential curve program, but use gradient elution by linear curve when it operates with
YLClarity software.
[Fig. 4] Gradient curve
2-6. Rinse port
When using buffer solution, salts are generated on back side of high pressure seal and these deposits
wear pump seal to cause shortening of seal life, which has bad effect on pump.
Rinse port enables to insert proper solvent in back side of high pressure seal to prevent salts from being
deposited and activated. Mixed solution(20% MeOH) of water and methanol is used as cleaning solution,
and life of seal is extended with lubrication action in general analysis..
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