WPI ISO-COP-2 Instruction Manual Download | Manualshive

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World Precision Instruments

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Summary of Contents for ISO-COP-2

Page 1: ...Instrumenting scientiﬁc ideas WORLD PRECISION INSTRUMENTS ISO OXY 2 OXELP Macrosensor for O2 Measurement Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 030316 ...

Page 2: ...Solution 95611 7325 100042 7326 100084 Start up Kit 95699 5435 600011 5377 600015 Higher detection limit available on request call for custom pricing Real time detection using electrochemical microsensors Measure nitric oxide from 0 3 nM to 100 µM Measure hydrogen peroxide 10 nM to 100 mM Measure hydrogen sulfide Measure glucose Measure oxygen from 0 1 to 100 Integrated system includes one tempera...

Page 3: ... ABOUT THIS MANUAL 1 INTRODUCTION 2 Notes and Warnings 2 Parts List 3 Unpacking 3 INSTRUMENT DESCRIPTION 4 Structure of the Sensor 4 OPERATING INSTRUCTIONS 5 Polarizing the Sensor 5 Calibration Procedure 5 SENSOR CARE AND MAINTENANCE 9 Durability and Handling 9 Storing the Sensor 9 Cleaning the Membrane 9 Sterilizing the Membrane 10 Replacing the Membrane Sleeve 10 ACCESSORIES 12 TROUBLESHOOTING 1...

Page 4: ...ii World Precision Instruments ...

Page 5: ...at can cause damage to equipment Please read these carefully This symbol indicates a WARNING Warnings alert you to actions that can cause personal injury or pose a physical threat Please read these carefully NOTES and TIPS contain helpful information Fig 1 The ISO OXY 2 sensor works with the TBR4100 TBR1025 and Apollo systems Fig 2 The OXELP sensor works with the ISO2 only ...

Page 6: ...o pass while blocking liquids ions and particulate matter Oxygen diffuses through the membrane The voltage applied to the sensor is held at 0 7V when the monitoring device is on and the sensor is properly connected The magnitude of the generated electrical current is determined by the rate of diffusion through the membrane The rate is proportional to the partial pressure of oxygen outside the memb...

Page 7: ...g shipment The tip of the sensor is protected by inserting it into a small plastic vial containing deionized water DIW to keep the membrane from drying out NOTE In some cases the sensor is shipped dry To open the package carefully cut the seals on either side of the sensor box Inside the box on top of the sensor are the important documents to read before handling the sensor including the instructi...

Page 8: ...g pair of working and counter reference electrodes Probe handle Locking cap attaches the sleeve to the probe handle When the sensor is fully assembled with locking cap and sleeve in place the internal electrode should press gently against the polymeric membrane which will be slightly stretched This ensures that the electrolyte diffusion is as thin as possible minimizing sensor response time NOTE O...

Page 9: ...ygen sensor in combination with a monitoring device amperometrically measures the concentration of oxygen in aqueous solutions and can be used short term 2 hours or less in a gas mixture Once the sensor is polarized It can be calibrated The polarized sensor should already be immersed in filling solution and plugged into the monitoring device The following example briefly describes the fundamental ...

Page 10: ...ed once It is set NOTE Do NOT let the sensor tip come into contact with a stir bar It could damage the membrane 4 Immerse the polarized sensor in the solution and secure it in an electrode holder such as WPI s Pro Guide WPI 47510 47520 47530 47540 or a micromanipulator The sensor tip should be immersed about 2 3mm into the solution Allow the sensor to remain in the solution at least five minutes a...

Page 11: ...o the baseline value The data should look similar to the graph in Fig 4 Fig 4 Calibration record showing established baseline at point 1 0 O2 reading at point 2 baseline re established at point 3 and 100 O2 reading at point 4 9 Construct a standard calibration curve using the recorded data Using a third party spreadsheet with graphing capability like Microsoft Excel it is possible to generate a li...

Page 12: ... Precision Instruments Fig 5 Calibration output The slope of this curve indicates the sensitivity of the sensor Once the sensitivity of the probe has been ascertained the sensor is ready to use experimentally ...

Page 13: ...care and by following the instructions below a membrane sleeve should last more than one month Use the following guidelines to maximize the life of the electrode STANDBY If the oxygen sensor is being used on a daily basis leave the monitoring device ON continuously with the sensor plugged in and its tip suspended in distilled water to maintain polarization SHORT TERM If the sensor is not to be use...

Page 14: ...nd the internal electrode assembly when doing so 3 Rinse the internal electrode with distilled water particularly the tip and let it soak for at least 15 minutes Be careful not to let water get up into the handle 4 Gently dry the sensor with a soft tissue Kimwipes Be sure to dry thoroughly the flat surface at the tip of the electrode After drying the current should stabilize fairly quickly to a lo...

Page 15: ... The current displayed on the meter at this time will be high or offscale 10 Suspend the tip of the newly assembled probe in 0 1M PBS buffer solution 11 After 10 15 minutes the current should no longer be offscale and will gradually decrease with time It may take up to one hour for the sensor current to reach a low stable value at which time it will be ready for use TIP The integrity of the new me...

Page 16: ... ISO2 Filling Solution electrolyte 7363 4 Enzol Enzymatic detergent 1 gal MF28G67 5 MicroFil electrolyte filling needle pkg of 5 The start up kit WPI 5377 contains everything needed to begin working with this sensor and the kit is highly recommended for first time users Fig 7 The kit includes Calibration bottle Two additional membrane sleeves Sold in packages of four with filling solution WPI 5378...

Page 17: ... baseline hasn t stabilized after an hour the polarizing solution may be contaminated Prepare fresh polarizing solution Use 0 1M PBS only External electrical interferences may be the problem Identify and isolate electrical interfer ences Calibration dataset is not linear The dilution factors may be incorrect See Calibration Procedure on page 5 Verify the procedure used Unsaturated solutions may be...

Page 18: ... zero when dry and extremely high in solution the sensor itself is probably working properly Install a new membrane sleeve See Replacing the Membrane Sleeve on page 10 TIP Do not reuse an old membrane because the membrane itself is stretched once it is used After it is removed it maintains the surface deformity and will not provide accurate test results if reused NOTE If you have a problem issue w...

Page 19: ...irect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Conceal...

Page 20: ...e com Germany Zossener Str 55 10961 Berlin Tel 030 6188845 Fax 030 6188670 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 29a No8 Dongfang Rd Pudong District Shanghai 200120 PR China Tel 86 21 6888 5517 E mail chinasales china wpiinc com Brazil Av Conselheiro Nébias 756 sala 2611 Santos CEP 11045 002 São Paulo Brazil Tel 013 406 29703 E mail info brazil wpiinc com Inter...

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