▪
Place a slide on the stage securely, beneath the spring-loaded
stage clips and align the specimen with the hole in the stage.
▪
Adjust the disc diaphragm so that the largest opening coincides
with the opening in the stage. This maximum aperture is generally
used at the lowest magnification.
▪
While looking through the eyepiece and using the course-focus
adjustment, slowly move the objective/body tube AWAY from the
stage/specimen until the image is clear.
▪
Rotate the 10X objective into place. While looking through the
eyepiece and using the fine-focus adjustment, clearly focus the
image, which now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.
▪
At this point, it may be beneficial to diminish the amount of light
entering the specimen. Decreasing the aperture of the disc
diaphragm will reduce glare and increase contrast in the image.
Use of the diaphragm will require experience. It is important to
select both the magnification and the degree of contrast that
renders the optimum detail in the observed specimen.
▪
Rotate the 40X objective into place and repeat the steps taken for
the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to slightly sharpen the focus adjustment once the initial image is
clearly viewed at low magnification.
▪
It will be useful to repeat these procedures until they become
second nature. Your familiarity with them will add to the
usefulness of the microscope and to the ease in interpreting the
information it provides.
NOTE: If, at any or all magnifications, it is not possible to establish a clear image,
verify: that the power supply is on or the light source optimized; that all optical
surfaces are clean; that the diaphragm is properly set to allow light to pass
through the specimen; and, that the travel stop has been correctly set according to
the procedure listed further in this manual.
FEATURES
WIDEFIELD POINTER EYEPIECE: The 10X widefield eyepiece, furnished with this
microscope, contains a built-in pointer. Position the pointer by rotating the
eyepiece.
FOCUS CONTROLS (TUBE TYPE): This microscope utilizes a fixed specimen stage.
Focus is achieved through use of the course focus adjustment knob and fine focus
adjustment controls. The knobs are located on the upper portion of the
microscope arm, above the stage. You should notice vertical movement of the
body tube when focusing an image. NOTE: Between the course focus knob and
microscope stand, on one side, is a tension collar. Clockwise rotation of the collar
increases tension of the course focus adjustment.
