Block Cooler V1.0
4
37 °C
In cell culture procedure to dissociate the cell with
Typsine/DNAase I solution in HBSS medium or resuspend with
DNase I solution only in DMEM medium.
The denature DNA steps in DNA extraction kit.
DNA/RNA probe preparing.
The incubation steps in cDNA synthesis.
Bacteria culture incubation or purification.
Enzyme reaction –
i.e: Restriction enzyme reaction and RNAase reaction by DNA
extraction or Flow cytometry of Apoptosis.
Incubate the plug at 37°C in Pulsed Field Gel Electrophoresis.
For gel digestion pre-treatment before MALDI-TOF MS analysis.
42 °C
The reverse transcription temperature by RT-PCR.
Enzyme reaction –
i.e: DNA ligase gene reaction for thermosensitive mutant
application.
The incubation procedure of phage purification.
Gene transformation incubation –
i.e: Transfer gene to E. Coli strain for expression.
55 °C
The steps required on cDNA synthesis.
Chemical reaction –
i.e: The gentle heating steps by DNA extraction kit solution.
Enzyme reaction –
i.e: RNAase reaction.
Gel digestion –
i.e: The oligonucleotides purify from SDS-PAGE gel.
