6
XSelect
XP
and XSelect Premier 2.5 µm HSS Columns
[ CARE AND USE MANUAL ]
b. Normal-phase Conditions
The HSS Cyano column can be used for both reversed-phase
separations as well as normal-phase separations. The column
is originally shipped in acetonitrile and is ready to use for
reversed-phase conditions.
If you intend to use the column for normal-phase
applications, you will need to condition the column
with the following procedure:
1. Flush the column with a minimum of 20 column
volumes of 100% methanol using a low flow rate to
avoid overpressuring the LC system. Refer to Table 1
for minimum solvent volume.
2. Flush the column with a minimum of 20 column
volumes of 100% isopropanol using a low flow rate
to avoid overpressuring the LC system. Refer to
Table 1 for the minimum solvent volume.
3. Flush the column with a minimum of 20 column volumes
of 100% dichloromethane using a low flow rate to avoid
overpressuring the LC system. Refer to Table 1 for the
minimum solvent volume.
4. Flush the column with the intended mobile-phase
conditions until a stable baseline is achieved.
c. Storage
For periods longer than four days at room temperature,
store reversed-phase XSelect HSS Columns in 100%
acetonitrile. For elevated temperature applications, store
immediately after use in 100% acetonitrile for the best
column lifetime. Do not store columns in buffered eluents.
If the mobile phase contained a buffer salt, flush the column
with 10-column volumes of HPLC-grade water (see Table
1 for common column volumes) and replace with 100%
acetonitrile for storage. Failure to perform this intermediate
step could result in precipitation of the buffer salt in the
column when 100% acetonitrile is introduced. Completely
seal column to avoid evaporation and drying out of the bed.
Note: If a column has been run with a mobile phase that contains
formate (e.g., ammonium formate, formic acid, etc.) and is then
flushed with 100% acetonitrile, slightly longer equilibration times
may be necessary when the column is re-installed and run again
with a formate-containing mobile phase.
d. Post Normal-phase Use
For rapid equilibration upon startup, it is recommended that
you store the HSS Cyano column in the mobile phase that is
commonly used for your normal-phase separation. Completely
seal the column to avoid evaporation and drying out of the bed.
IV. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
The eCord Intelligent Chip provides the history of a column’s
performance throughout its lifetime. The eCord will be
permanently attached to the column to assure that the
column’s performance history is maintained in the event that
the column is moved from one instrument to another.
At the time of manufacture, tracking and quality control
information will be downloaded to the eCord. Storing this
information on the chip will eliminate the need for a paper
Certificate of Analysis. Once the user installs the column,
the software will automatically download key parameters
into a column history file stored on the chip. In this manual,
we explain how the eCord will provide a solution for easily
tracking the history of the columns, reduce the frustration of
paperwork trails, and give customers the reassurance that a
well-performing column is installed onto their instruments.
b. Installation
Install the column into the column heater. Plug the eCord
into the side of the column heater. Once the eCord is inserted
into the column heater the identification and overall column
usage information will be available allowing the user to access
column information on their desktop.
Figure 3. eCord Intelligent Chip.
eCord Intelligent Chip
Figure 4. eCord inserted into side of column heater.
eCord inserted into
side of column heater
