Waters ACQUITY, Overview And Maintenance Manual

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Table C–2: Solvents used to prepare mobile phases (KAD) (continued)
Solution Concentration
Ethanol 100%
Isopropanol 100%
Propanol 100%
Formic acid ≤0.5%
Acetic acid ≤1%
Trifluoroacetic acid (TFA) ≤0.2%
Ammonium hydroxide ≤1%
Ammonium formate ≤50 mM
Ammonium acetate ≤50 mM
Ammonium bicarbonate ≤50 mM
Tetrahydrofuran (THF) ≤15%
C.3.3 Solvents used infrequently
The following reagents are used less frequently with electrospray ionization than those listed in
the previous table, because they decrease sensitivity. However, they are often required for
suitable chromatographic resolution.
Note:
At the concentrations specified below, the reagents are not expected to adversely affect
the performance of the materials shown in the list of items exposed to solvents in Items exposed
to solvent . However, you must keep concentrations to the lowest levels that maintain
chromatographic resolution.
Table C–3: Solvents used infrequently
Solution Concentration
Diethylamine (DEA) ≤ 0.5%
Triethylamine (TEA) ≤ 0.5%
Hexafluoroisopropanol (HFIP) ≤ 5%
Hexyl Ammonium Acetate ≤ 50 mM
June 15, 2017, 715003956 Rev. F
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