• To ensure reproducibility, overfill the loop by three times the loop volume. Overfilling
flushes out excess solvent from previous infusions, and ensures that sample is
introduced to the QDa consistently.
• When loading samples, consider the miscibility of the sample solvent and the carrier
solvent. Dilute the sample in the carrier solvent before filling the loop, if necessary.
Result:
The loop fills with sample ready for introducing into the QDa for analysis.
4.
Start the QDa acquisition using the QDa software.
See also:
The instrument software Help.
5.
Manually actuate the valve by pressing the
Up arrow
(
) on the front panel to move the
valve from position 1 to position 2.
Result:
Sample is injected through the loop into the QDa.
6.
To prepare for the next injection, manually actuate the valve by pressing the
Down arrow
(
) on the front panel to move the valve from position 2 to position 1.
See also:
•
Manually operating the diverter valve
•
Programming the diverter valve operation
E.4 Diverter valve maintenance procedures
This section provides the maintenance guidelines and procedures necessary to maintain the
device's performance.
Clean the diverter valve when the performance reduces to unacceptable levels. Replace diverter
valve components if they become irretrievably damaged.
E.4.1 Cleaning the diverter valve
Required material
• Chemical-resistant, powder-free gloves
• Suitable vessel in which to immerse rotor seal and stator when cleaning
• 9/64-inch hex wrench supplied with the ACQUITY Diverter Valve Assembly Kit
• HPLC-grade (or better) 1:1 methanol/water
• Ultrasonic bath
• Source of oil-free, inert gas for drying (for example, nitrogen)
June 15, 2017, 715003956 Rev. F
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