Waters 2475, Operator'S Manual, Page: 90 / 228

3-18 Using the Detector
Preparing to start a run
You must set up a run before you make fluorescence measurements. To start a
run, you can press Run/Stop or trigger the detector’s operation via the inject-
start terminals on the rear panel. When you start a run, the shutter opens
automatically and the detector performs an auto-zero function (when the
function is enabled).
You must select Auto Zero-On-Inject for the detector to automatically auto
zero (see page 3-19 ).
While under Empower or Millennium
32
control (see page 3-29 ), the shutter
closes when the detector finishes a run, and the run timer stops and then
resets. If you run the detector manually, you can close the shutter by pressing
Reset or waiting until the run ends.
To avoid the automatic opening and closing of the shutter, you can disable the
idle mode by checking the Selection box in the Configuration screen (see
page 3-4 ).
Setting up a run
After you press HOME to return to the home screen and select a channel mode
( λ or λλ ), you can set up the detector for a run. Before beginning a run,
however, you must select the channel mode, and program the following
parameters:
• Operating wavelength pairs
Tip:
The emission λ setting (e λ ) must be at least 10 nm above the
excitation
λ
setting (x
λ
).
• Gain
• Output sensitivity, EUFS
• Filter type
• Time constant
• Analog output type
• Data units
If you want to perform other functions during a run, you must program other
parameters. “Accessing primary and secondary functions” on page 3-19 and
the table titled “Primary and secondary function (method) parameters” on