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Page 1: ...W Wa ar rn ne er r I In ns st tr ru um me en nt ts s P Pi ic co o l li it te er r I In nj je ec ct to or r M Mo od de el l P PL LI I 1 10 0...
Page 2: ...oocyte injection Features for the PLI 10 include 9 Low cost 9 Femtoliter to microliter injections 9 Digital readouts for injection pressure time and count 9 Reliable optically encoded circuit for inj...
Page 3: ...upplies 10 Optional Equipment for Microinjection 10 Micropipettes 10 Distinguishing Parameters 10 Choosing the Right Pipette 10 General Considerations 11 Injection Pressure and Time Pulse 12 Gas Usage...
Page 4: ...ty rather than complexity we welcome any feedback you may wish to provide Warner Instrument product numbers are presented using bold type References to specific controls within a block are specified u...
Page 5: ...reproducibility Injection time is set using an optically encoded circuit which permits fine and coarse settings from a single dial knob The system trigger timer can be controlled by the front panel p...
Page 6: ...initiating injection from an external trigger source A positive TTL pulse of is required baseline 0 V trigger 5 V lasting 10 ms J INJECTION TIME SETTING DIAL This dial controls the injection time sett...
Page 7: ...1278 as an P REMOTE INJECT foot switch input connector An optional foot switch PLI FS can be connected to the instrument Pushing the foot switch triggers an injection for the preset time and pressure...
Page 8: ...ill allow fine adjustment of injection time in tenths of seconds 7 Turn the INJECTION PRESSURE set knob Item K to set the desired injection pressure displayed in Item E Pressure can be set and monitor...
Page 9: ...remove the pipette from the holder and power the unit off The air gas input should also be turned off at the source ADDITIONAL INFORMATION Note The following material is excerpted from the user s manu...
Page 10: ...ecrease in taper angle longer taper would decrease the delivery rate about 10 The extreme sensitivity of delivery rate on tip diameter makes it important to have a reproducible pipette puller If you u...
Page 11: ...n The microscope is focused on the cell s top surface and the tip comes into focus just before insertion Again shearing forces are avoided Suitable bends can be made with a microforge a simple way to...
Page 12: ...he pipette begins to flow out the tip opening The pressure shown on the LCD can now be used as the initial injection pressure setting Adjust the injection pressure and timing to obtain the desired inj...
Page 13: ...80 20 1 1 3590 40 3 59 30 1 1 5385 60 5 39 40 1 1 7180 80 7 18 50 1 1 8976 00 8 98 60 1 1 10771 20 10 77 1 1 5 22 44 0 02 10 1 5 224 40 0 22 20 1 5 448 80 0 45 30 1 5 673 20 0 67 40 1 5 897 60 0 90 50...
Page 14: ...in Power Supply universal input voltage 90 to 264 VAC Weight 2 3 kg Dimensions 89 x 215 x 175 mm H x W x D Warranty One year parts labor Ordering Information Model number Description Order number 64 1...
Page 15: ...stions regarding service be referred to our Technical Support Department Normal business hours are 8 30 AM to 5 00 PM EST Monday through Friday We are located at 1125 Dixwell Avenue Hamden CT 06514 We...
Page 16: ...92 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Manufacturer s Name Warner Instruments LLC Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Equip...
Page 17: ...urer s Name Warner Instruments LLC Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Equipment Description Pico liter injector Safety requirements for electrical equipment fo...
Page 18: ...ments 1125 Dixwell Avenue Hamden CT 06514 USA 203 776 0664 800 599 4203 Fax 203 776 1278 Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 USA 203 776 0664 800 599 4203 Fax 203 776 1278 Rev 09071...
