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4. Gel Preparation
Place the gel casting stand on a level surface
and place the divider and desired gel trays
into the proper positions in the stand.
Either one long gel tray, or two short trays can
be inserted into the casting stand (see
diagrams on right).
Note: If the gel tray is not level, the thickness
of the gel will not be uniform and DNA
migration may be uneven.
Insert the comb(s) into the gel casting
stand as per image on the right. Multiple
combs can be inserted depending on the
number of samples and desired run
distance.
Note: when using the fine-toothed
comb, the flat side of the well comb
should be facing the direction of
DNA travel. See figure below.
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