Velp Scientifica
– UDK139 Operating Manual
10004762/B5
115
Steam power: 50 %
Position the test tube containing 10 ml of sample into the distillation unit UDK and
start the distillation.
Push START to begin the distillation.
About 200 ml of distillate are collected into a 500 ml volumetric flask.
Blank: 10 ml of distilled water Type II
Dilute the distillation solution to 500 ml using water type II and transfer the solution
into a 1000 ml becker.
Add 25 ml of ammonium chloride solution 2 % by using a pipette and adjust the pH to
10.0 ± 0.2 with some drops of ammonia 25 %.
Transfer the solution into a 500 or 1000 ml separatory funnel and add 3.0 ml of
Aminoantipyrine solution and 3.0 ml of potassium ferricyanide solution. Shake the
separatory funnel and after 5 minutes add 25 ml of chloroform with a pipette.
Shake vigorously for about 2 minutes and let the organic phase settles.
Filter chloroform extract through a filter paper and collect the filtrate in a 100 ml flask.
Transfer the filtrate solution in a 50 mm glass cuvette and read the extinction values
at 460 nm, against distilled water.
Note:
In order to calculate the phenol concentration, create a calibration curve by using
phenols solution diluted in the following range: 0.005-0.100 mg /l phenols.
Dilute different amounts of the phenols diluted solutions to 500 ml using water type II
and proceed the chloroform extraction as indicated above. Record the extinction
value corresponding to the different concentrations.
References:
-
APAT CNR IRSA (Water Research Institute), Italy, method n° 5070A1
- EPA method n° 9065
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