Fluid Imaging
Operating Principles
Rev. D
Dimension 3100 Manual
165
Focus Surface Procedure
1. Use the
Stage
>
Focus Surface
command to bring the sample into focus on the video camera
monitor, as described in
2. Move the X-Y sample stage to bring an area of interest under the AFM cantilever tip.
10.5.14 Cantilever Tune (TappingMode Only)
This section describes the steps required to find the resonance peak of the cantilever and adjust the
oscillation voltage so the cantilever vibrates at an appropriate amplitude. A range of oscillation
frequencies are applied to the cantilever to determine the frequency which produces the largest
response (the resonance frequency). In most instances, the resonance peak has a sharp Gaussian
distribution but at times the peak can be ragged. The system tolerates some deviation in the shape of
the peak.
Manual
Cantilever Tune
is the counterpart to the step used in standard (air) TappingMode to find
the resonant frequency of the cantilever. In liquid, however, the cantilever resonance is largely
CAUTION:
Use extreme caution moving the X-Y stage if the sample holder
has a lip that extends above the sample surface (i.e. petri dish). The
SPM head can be destroyed if the sample stage is moved such that
the tip holder crashes sideways into the lip of your sample holder.
Also, check that the position of the lip of the sample holder will not
interfere with the movement of the Z-stage.
ATTENTION:
Il est recommandé de déplacer avec une extréme vigilance la
platine quand le support d’échantillon présente un rebord qui se
trouve à une hauteur supérieure à mesurer (comme une boîte de
Petri). La tête de mesure peut être endommagée si le support de
pointe vient heurter le rebord du support d’échantillon lors d’un
déplacement de la platine. De plus, il est impératif de vérifier que
le rebord du support d’échantillon ne viendra pas perturber le
mouvement vertical du moteur de la tête de mesure.
VORSICHT:
Seien Sie extrem vorsichtig mit XY-Bewegungen des
Probentisches, wenn der Rand Ihrer Probenbefestigung über die
Probenfläche hinausragt (wie zum Beispiel bei einer Petrischale).
Der SPM-Kopf kann zerstört werden, falls der Probentisch derart
bewegt wird, daß der Spitzenhalter seitlich gegen den Rand der
Probenbefestigung schlägt. Versichern Sie sich außerdem, daß der
Rand Ihrer Probenbefestigung bei Bewegung der Z-
Verschiebeeinheit nicht im Weg ist.
Summary of Contents for Dimension 3100
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