Fluid Operation
Lysozyme on Mica—A Model Procedure for Protein Binding
144
MultiMode SPM Instruction Manual
Rev. B
9. After the fluid cell has been flushed with buffer solution, reclamp the drain line. This is
important for low-noise, low drift imaging. The sample is now ready for TappingMode
imaging. A good TappingMode image of lysozyme protein on mica is shown in
Figure 8.6b
TappingMode image of lysozyme in buffer solution using above sample preparation (Scan size =
500nm).
Note:
It is also possible to prepare samples inside the
fl
uid cell by
fl
owing the protein
solution through the
fl
uid cell. In this case, it may be helpful to engage the tip in
contact mode with a zero
Scan size
to protect proteins from binding to the tip.
