Van Guard 1400INi Series, Operation Manual

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4'1:D1ntroduction
Thank you for purchasing this VanGuard microscope. With the user in mind, VanGuard microscopes are built
from modern designs and should provide a lifetime of reliable performance. Before using this microscope it must
be properly setup, which requires some familiarity with the microscope. For this reason we recommend you read
this entire manual carefully before setting up and using the instrument.
14001Ni Series Inverted Microscopes
Vanguard 1400INi Series inverted microscopes
are suited for observation of blologlcal cultures
or specimens within well plates, petri dishes,
slides and vials.
Viewing Head.
Rotatable trinocular (Seidentopf)
head is inclined at 30
°
. All models feature
interpupillary and dioptric adjustment. The trinocular
head features a sliding main prism (80/20 spin) to
provide full-time imaging when the vertical tube is
in use (80% of the image to the vertical tube and
20% to the eyetubes).
Eyepieces. 1 OX uttra-widefield (DIN) with a field
diameter of 22mm.
Nosepiece. Quintuple, ball-bearing nosepiece with
high-grade lubricant and posnive stops.
Objectives. Plan achromatic, infinity corrected
objectives come standard on both models.
Objectives are made to DIN standards and are
optically coated.
Stage. The fixed stage measures 160 x 250mm and
300 x 250mm with stage extensions/mechanical
stage attached. Mechanical stage accepts 96
well microplate and included stage inserts:
Terasaki/65mm petri dish, 1 x 3" slide/54mm petri
dish, 35mm petri dish. Motion of mechanical stage
is controlled by a right-hand, low-posttion coaxial
control and is driven by a rack and pinion system.
Focusing movement. Coaxial, ultra-low posttion
coarse and fine focus controls feature a 40mm
focusing range and are graduated to 2 microns per
division. Fitted with a tension adjustment.
Condenser. Brightfield models come with a 0.30
N.A. condenser. Phase contrast models come with
a 0.30 N.A. condenser with phase annulus slider
for 1 0X/20X, and 40X; also has a open setting for
brightfield work. All condensers are factory centered
and aligned for ease of setup and include an iris
diaphragm, and a slide-in fitter holder.
Illumination. 30W variable quartz halogen light
source. Comes with blue, green (model 1493INi
only), and dispersion filters. 1 A, 250V fuses.
Body. Cast-metal, ergonomic body with stain­
resistant enamel finish.
[ Table of Contents
Introduction ........................................................................
2
Parts & Accessories
lnduded Parts ........................................................................ 3
OptionalAccessories ............................................................. 4
Microscope Diagrams ......................................................... �
Setup
Assembly........................................................................ 7-12
Using Your 1400INi Microscope
Power SwitchNariable Lighting Control ................................. 12
lnterpupillary and Diopter Adjustment... ................................... 9
Focusing and Mechanical Stage Mechanisms ........................ 13
Using the Brightfield/Phase Contrast Condenser .................... 13
Using Filters.......................................................................... 11
Using the Camera Port ........................................................... 11
Maintenance
Aligning the Phase ContrastAnnulus Rings ............................. 1
o
Replacing the Lamp .............................................................. 14
Replacement lamp Information ............................................. 14
Replacing the Fuses.............................................................. 15
Replacement F use Information ............................................. 15
Caring for your 1400INi Microscope ........................................ 16
Troubleshooting ....................................................................
18
Specifications ..................................................................
19
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4'1:Dspecifications
Viewing Head:
Viewing Head Type:
Head Rotation:
Head Inclination:
Sliding Prism:
lnterpupillary Adjustment:
Dioptric Adjustment:
Eyepiece Magnification:
Eyepiece Field Diameter:
Nose piece:
Brightfield Objectives:
(Plan Achromatic, Infinity Corrected)
Phase Contrast Objectives:
(Plan Achromatic, Infinity Corrected)
Stage Dimensions:
Stage Motion:
Stage Movement Range:
Sample Clearance (Brightfield):
Focusing Movement:
Focusing Graduation:
Brightfield Condenser (1491INi):
Trinocular
Seidentopf
360
°
30
°
100% or 80/20% Split
48-75mm
-6 to +6
1 OX High Eyepoint, Ultra-Widefield
22mm
Quintuple
4X
(0.10 N.A., 25.2mm W.D.]
10X (0.25 N.A., 11mm W.D.]
20X [0.25 N.A., 6mm W.D.]
40X (0.60 N.A., 3.2mm W.D.]
10X (0.25 N.A., 11mm W.D.]
20X (0.25 N.A., 6mm W.D.]
40X (0.60 N.A., 3.2mm W.D.]
160mm x 250mm
Right-hand Coaxial Control/Rack & Pinion Drive
78 x 120mm
75mm [Standard]
150mm [With condenser lens removed]
Coaxial Coarse & Fine Controls
2 microns Per Division
0.30 N.A. Condenser with Iris Diaphragm
Phase Contrast Condenser (1493INi): 0.30 N.A. Condenser with Iris Diaphragm & Annulus Slider
Phase Centering Tool: Telescoping Eyepiece [1493INi only]
Illumination:
Fuse:
Voltage:
Base Dimensions:
Overall Dimensions:
Weight:
30W, 6V Variable Quartz Halogen
1A, 250V (2 ea)
110V [Standard], 220V [Optional]
320mm x 245mm
515mm (L) x 300mm (W) x 475mm (H)
11kg
19
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