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UVP ChemiDoc-It2, Installation And User Instructions Manual

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UVP ChemiDoc-It2 Installation And User Instructions Manual Download Page 3

 

3 

 

81-0220-01 Rev R 

7.5

 

Using the UV Gel Viewer Window ........................................................... 24

 

8

 

Capturing Images and Using Templates ....................................................... 25

 

8.1

 

Image Focusing ....................................................................................... 25

 

8.2

 

Capturing Images .................................................................................... 26

 

8.3

 

Using Templates ...................................................................................... 27

 

9

 

Maintenance, replacement parts/accessories .............................................. 29

 

9.1

 

Care and cleaning ................................................................................... 29

 

9.2

 

Replacement parts and accessories ....................................................... 29

 

9.3

 

Troubleshooting ....................................................................................... 32

 

10

 

Disposal ............................................................................................................ 32

 

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Summary of Contents for ChemiDoc-It2

Page 1: ...tion and User Instructions UVP LLC Ultra Violet Products Ltd 2066 W 11th Street Unit 1 Trinity Hall Farm Estate Upland CA 91786 Nuffield Road Cambridge CB4 1TG UK Phone 800 452 6788 Phone 44 0 1223 420022 Fax 909 946 3597 Fax 44 0 1223 420561 Web Site www uvp com ...

Page 2: ...t Focus Target 10 4 6 Optional Equipment 11 4 7 Thermal Printer 11 4 8 UV Handheld Lamps for Epi Overhead Lighting 11 4 9 LED White Light Plate 11 4 10 Converter Plates 11 4 11 VisionWorks Acquisition and Analysis Software 12 5 Set Up 13 5 1 Scope of supply 13 5 2 Connecting the power cables 13 5 3 Installing emission filters 14 5 4 Camera Setup 14 6 Software Installation 20 6 1 Installing VisionW...

Page 3: ... Capturing Images and Using Templates 25 8 1 Image Focusing 25 8 2 Capturing Images 26 8 3 Using Templates 27 9 Maintenance replacement parts accessories 29 9 1 Care and cleaning 29 9 2 Replacement parts and accessories 29 9 3 Troubleshooting 32 10 Disposal 32 ...

Page 4: ...ctographs and signal words The type and source of the danger are stated together with notes on preventing the danger The meaning of the pictographs and signal words used is explained in the chapter Safety instructions 1 2 Intended use The GelDoc It2 Imager is a high resolution imager capable of capturing documenting and analyzing fluorescent gel images The ChemiDoc It2 Imager is designed as a high...

Page 5: ...4 GB preferred 200 MB of available hard disk space for the program more for data CD ROM drive USB ports The computer must come equipped with a minimum of three USB2 0 ports Additional peripherals mouse keyboard printer etc may require additional USB ports Color monitor supporting at least 1024 x 768 resolution and 16 bit or better 24 bitor 32 bit color is strongly recommended IMPORTANT For 21 CFR ...

Page 6: ...bient The 815 CCD has a peak quantum efficiency of 50 and is also capable of binning from 1 x 1 to 8 x 8 All camera settings are factory pre set for optimum performance when viewing gels films or membranes under low light level conditions Contact Technical Support prior to making any adjustments to the camera settings Lens Kit Motorized or Manual The lens used comes in two configurations The motor...

Page 7: ...carefully before operating Observe all safety notes listed in this user manual Symbols and signal words used The user manual uses the following symbols and signal words to indicate safety hazards warnings and instructions General warning Caution ultraviolet radiation Important hints or useful information ...

Page 8: ...n the back of the unit must be ensured Disconnect the power supply before servicing the system For UV protection and to extend the life of the UV transilluminator the system incorporates a customizable transilluminator shutoff timer built into the software For additional information refer to Section 4 4 Caution Do not override the UV safety switch located on the darkroom without the use of protect...

Page 9: ...without cover only with different camera bracket GelDoc It 2 ChemiDoc It 2 Imagers Camera and Lens Kit actual configuration may be different than shown in the photo Lighting and UV Timer Switches Epi Overhead White Light Four Position Emission Filter Selector UV Safety Interlock Switch UV Safe Gel Viewer Window Roll Out Transilluminator LED White Light Plate Optional Access port for optional BioLi...

Page 10: ...ts protein gels colony plates etc in order to produce brighter images 4 4 Transilluminator The GelDoc It2 and ChemiDoc It2 Imagers can accommodate UVP s Benchtop and FirstLight transilluminator models UVP offers a variety of transilluminator configurations including Benchtop models with multiple wavelengths and variable intensities as well as the highly uniform patented FirstLight transilluminator...

Page 11: ...nd used as standalone handheld lamps as seen in the image to the right UV Lamp 4 9 LED White Light Plate The LED White Light Plate emits high uniformity with less than 5 coefficient of variance CV Plug the LED White Light Plate directly into the power supply within the system darkroom or store it in the exterior dark room side pocket when not in use LED White Light Plate 4 10 Converter Plates An a...

Page 12: ...software features image enhancement complete analysis tools and reporting capabilities and is ideal for users who require image analysis functions in addition to the standard image acquisition capabilities IMPORTANT VisionWorks is included as standard Software must be installed on an external computer For installation instructions please see next chapter as well the separate software instructions ...

Page 13: ...ust or high temperatures Keep the equipment away from motors or any other large magnetic equipment apparatus This system is designed for indoor use only 5 2 Connecting the power cables 1 Inside the darkroom place the transilluminator on the roll out tray Connect the transilluminator to one end of the jumper cable Connect the other end of the jumper cable to the interior of the darkroom Turn the UV...

Page 14: ...kroom Additional and replacement emission filters are available through UVP Refer to the Replacement Parts and Accessories section of this manual for ordering information 5 4 Camera Setup High sensitivity scientific grade cameras are designed for use with UVP s imagers This section covers the components and steps required to install cameras The following cameras are covered in this manual refer to...

Page 15: ...hat a light tight seal is made between the end of the lens and the rubber gasket beneath the darkroom bracket 5 Plug the additional power cables to an external power source Do not plug the camera USB into the computer until VisionWorks LS has been in stalled Darkroom bracket Brass thumb nut Camera bracket Camera USB cable Camera and lens assembly Rubber gasket ...

Page 16: ...ee cameras interchangeably Camera Installation The photograph shows the parts required for assembly of the 815 CCD and 515 CCD cam era kits with the motorized lens on the Imager Note The image above shows the motorized 42 5mm f 0 95 lens Another lens and or lens controller may be shipped with the system but will be installed in the same manner Camera power supply Camera and lens Lens controller po...

Page 17: ...se the black thumb screw s to tightly secure the camera in place attaching it through the hole at the top of the bracket 5 Plug the serial cable from the motorized lens into the lens controller 6 Plug the camera cable into the top of the camera Do not plug the other end of the USB cable for the lens controller and camera into the computer until VisionWorks software has been installed Note When loo...

Page 18: ...ens by pulling straight off Do not unscrew the lens cap as it will simply pull off 2 Follow steps 1 4 from the Camera Installation section under 5 4 2 Cameras with Mo torized Lens 3 Plug the camera cable into the top of the camera but do not plug in the other end of the USB cable until VisionWorks software has been installed Camera and lens Camera power supply Camera power cable USB camera to PC D...

Page 19: ...onnect the USB cable to the PC connection port on the lens control box Leave the other end of the USB cable unplugged until installation of VisionWorks is complete 7 Connect the power supply to the lens control box and into the power outlet Hook for the Lens Control Box Power switch for the Lens Control Box Power supply on the Lens Control Box PC Connection on the Lens Control Box ...

Page 20: ...se On the Fly activation Activation can aso be done using a smartphone Activation can also be done at REG UVP com using any other web connected device A Config ID will be needed for this option and can be found by clicking Next after selecting On the fly activiation It will be displayed under Configuration ID 3 Click Next to continue 4 The Already have an activation ID option is useful when reload...

Page 21: ... Installing Camera Drivers 1 The software must be installed for the camera drivers to be installed Open the Vision Works software suite 2 Plug the USB cable from the camera into the USB port At this time the lens controlbox USB or lens controller USB should also be plugged into the computer 3 The computer should display Found New Hardware The drivers will automatically install Note The installatio...

Page 22: ...olet power switches are three way rocker switches Selecting the upper switch position will turn on the corresponding overhead illumination Selecting the lower switch position will turn on the corresponding transillumination Finally selecting the middle switch position will turn off the lighting 7 1 1 UV Timer For UV light protection and to extend the life of the UV transilluminator there is a ten ...

Page 23: ...nt of the transilluminator unit is in the ON position IMPORTANT Do not attempt to use both the White Light and Ultraviolet transillumination sources simulta neously as system damage may occur For UV light protection and to extend the life of the UV transilluminator there is a ten minute transilluminator UV Timer installed in the system See UV Timer for more information Refer to the Transilluminato...

Page 24: ...oom door To open the Window press firmly on the top of the Window cover to release the pressure sensitive clasp and open the viewer The Window glass is UV blocking while providing a clear view to the transilluminator surface for sample viewing without opening thedarkroom door IMPORTANT Close the UV Gel Viewer Window prior to capturing a light sensitive image such as a chemi luminescent blot ...

Page 25: ...view the image Adjust the camera settings including exposure time to enhance the image of the sample To adjust the settings go to the Acquisition Action Tab and click on the Camera Menu Button Adjust the exposure time from the Exposure Time section of the Camera Menu 3 Rotate the lens f stop adjustment top ring so that the image is bright enough to be seen on the screen 4 Rotate the lens focus adj...

Page 26: ...mi tray transilluminator or white light plate 2 For a chemiluminescent sample turn off all darkroom lighting place the black chemi tray on top of the transilluminator and place the sample on the tray For a fluorescent gel sample turn on the UV transilluminator 3 Adjust the capture settings in VisionWorks 4 Click the Capture button 8 2 1 Recommended Settings for Short Exposures and Focusing 1 Incre...

Page 27: ... Chemi Blot with Fast Capture and Lower Resolution For this application use a preview binning of 8 x 8 a capture binning of 4 x 4 and an exposure time of 15 sec This setting is useful for quick screening the time can be increased or decreased depending on the intensity of the signal from the blot For better resolution set the preview binning to 8 x 8 the capture binning to 2 x 2 and the exposure t...

Page 28: ...oor 5 Immediately start previewing with the Chemi Blot with Fast Capture and Lower Resolu tion template Let the blot develop inside the darkroom while watching the preview When approaching maximum signal which should take 2 to 3 minutes use the Chemi Blot with Lower Capture and Higher Resolution template to capture the image 6 Light emission will drop off quickly so capture several images as quick...

Page 29: ...y cleaning with a damp cloth is allowed Alcohol and glass cleaner detergents attack the plastic and damage the UV protection shield 9 2 Replacement parts and accessories Replacement parts and accessories are listed below Only authorized UVP service personnel should perform repairs or replacements other than specified in theseprocedures For replacement parts of the transilluminator please refer the...

Page 30: ...6cm 38 0191 01 Visi Blue Converter Plate 21x26cm 38 0200 01 Gel Accessories Gel Cutter 85 0002 01 Gel Ruler 85 0003 01 Gel Scooper 85 0006 01 Gel Tray small 85 0007 01 Gel Sentry DNA Preparation Plate 97 0076 01 Fluorescent Standard Step Tablet 33 0014 02 Protective Equipment Spectacles UV Blocking UVC 303 98 0002 01 Goggles UV Blocking UVC 503 98 0002 02 Faceshield UV Blocking UVC 803 98 0002 04 ...

Page 31: ...Maintenance replacement parts accessories 31 81 0220 01 Rev R ...

Page 32: ...dition to the Ultraviolet Trans power switch on the front of the darkroom the transilluminator itself has a power switch Make sure that the green transilluminator power switch located on the front of the transilluminator is in the ON position 2 For UV protection and to extend the life of the UV transilluminator there is a ten minute transilluminator shutoff timer installed in the system The timer ...

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