Unico S-2150 Series User Manual Download Page 34 | Manualshive

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Unico S-2150 Series User Manual Download Page 34

34

3.1.4

WL Calibration (Wavelength Calibration)

At “System Setup” choose “WL Calibration” to recalibrate the system and the wavelength.

(If you decide not to recalibrate the wavelength press

【

ESC

】

to return back to

“System Setup”.)

a)

Recheck Dark Current

Dark current

Calibration λ...

546nm

b)

Move back to initial position

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Summary of Contents for S-2150 Series

Page 1: ...S 2150 SERIES SPECTROPHOTOMETER USER S MANUAL V 2 0 United Products Instruments Inc 182 E Ridge Road Dayton NJ 08810 732 274 1155 FAX 732 274 1151 www unicosci com Rev S2150 5 0...

Page 2: ...otometer Initialization 8 BASIC MODE T Abs Measure 11 Quantitative Test 14 Define Establish Test Method 15 By Standards 15 By Known Factors 23 Run Tests 28 System Setup 31 Edit clock 31 Dark Current 3...

Page 3: ...lling and using the instrument and its accessories The S2150 Series S 2150 visible and S2150UV UV Vis should be operated by appropriate laboratory technicians Electrical Before switching on the appara...

Page 4: ...gth range of 325nm to 1000nm for S 2150 and 200nm to 1000nm for model S 2100UV S 2150 is ideal for measurements in the visible wavelength region of the electromagnetic spectrum and S 2150UV in ultravi...

Page 5: ...in good condition Packing List Unless otherwise specially ordered the spectrophotometer package should include the following items Description ________________________ Quantity Spectrophotometer 1 Pow...

Page 6: ...at you have received everything in good condition 2 Place the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as p...

Page 7: ...EL Clear or delete SET Set wavelength 0Abs 100 T Blank Set 0Abs and 100 T or establish baseline LOAD Load saved curve MODE Select type of measurement ESC Escape or back to previous screen ENTER Confir...

Page 8: ...the Power Switch IO on the back of the instrument The instrument will automatically run a self initialization check The screen displays sequentially the checking status Booting System Initializing Ch...

Page 9: ...ta and the display will move to the main menu and ready to use If you select Yes the instrument will go through system calibration Below are some displays showing system calibration process Note If pr...

Page 10: ...6nm Warm up 15 min System calibration UNICO Instrument Ltd The instrument is ready for use Below is the Main Menu 2 2BASIC MODE T Abs or Energy Measurement 2 2 1 Use arrow button to highlight BASIC MO...

Page 11: ...rgy type is selected the display will show the energy counts as shown below 2 2 3 To reset wavelength press SET button The display first shows the current wavelength Enter the desired wavelength as sh...

Page 12: ...by filling a clean cuvette with distilled or de ionized water or other specified solvent Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid 2 2 5 Fit the blank cuvette int...

Page 13: ...2 10 Put the sample cuvette s in the sample compartment Close the lid 2 2 11 The current sample test result is displayed on the screen 2 2 12 Press ENTER to confirm and log the result Up to 20 test re...

Page 14: ...nd the samples in the next available position This will shorten the time to read samples and minimize the sample handling opening and closing the sample compartment lid etc To print the result press P...

Page 15: ...e main menu Use to choose the function and press ENTER to confirm your selection 2 3 1 Create New Curve At Quantitative use to Create New Curve and press ENTER to confirm your selection You can establ...

Page 16: ...he wavelength selection Then the type of curve needs to be selected There are two kinds of curves Linear or Linear through zero Press to choose and press ENTER your curve selection The next step is to...

Page 17: ...standard can be measured The average will be used for final calculation Use the numerical key to enter the desired times of measurement for each standard concentration DO NOT press ENTER yet Please in...

Page 18: ...sample solutions for each standard concentration Follow the step by step instruction on the LCD screen to measure all the standard samples Enter the concentration value of the first sample solution o...

Page 19: ...rocedures for the third standard sample solution of the No 1 standard After the last standard sample solution of the No 1 standard is measured the LC will show Confirm Y with Y highlighted The instrum...

Page 20: ...20...

Page 21: ...d the curve will not be saved and the curve will be displayed on the screen Use and to switch display between the curve and the equation Press ENTER to start sample test The curve will be used for one...

Page 22: ...of the saved curve If you decide to save the in any other open slot or want to replace an existing saved curve use the and to highlight that open slot or saved curve press ENTER to save Up to 200 curv...

Page 23: ...standard curve will be displayed regardless of your choice to save or not save the curve Use and to switch display between the curve and the equation If you choose not to save the curve before and no...

Page 24: ...se to scroll the unit list ppm ppb ng ul ng ml g l mg l Press ENTER to confirm the unit selection Next is to select the wavelength use 0 9 numerical keys to enter the desired wavelength i e 500nm Pres...

Page 25: ...B value Then you need to decide to save the curve or not Please refer to Save Curve and Replace previously saved Curve described in By Standards 2 3 4 Edit Curve At Quantitative use to highlight Edit...

Page 26: ...o cancel editing before measuring the new standards 2 3 5 Delete Curve At Quantitative use to highlight Delete Curve Press ENTER to confirm the selection Use to highlight the Curve to be deleted and p...

Page 27: ...e to confirm Are you sure NO is displayed Press ESC to stop deleting process If you are absolutely sure you want to delete the curve switch Yes using or button Press ENTER and the curve will be perman...

Page 28: ...ER to get into Load Curve screen Press ENTER to load the highlighted curve and run test 2 3 7 Load Curve to Favorite Tests At Quantitative use to select Load Curve Press ENTER to get into Load Curve s...

Page 29: ...ly used curves At Quantitative use to select Favorite Tests Press ENTER to confirm your selection Select the desired curve in the favorite tests list and press ENTER to run test If you decide to remov...

Page 30: ...ce into the cuvette holder in the optical path Press 0A 100 T to blank 0 562 A 0 341 500nm Blanking No Abs ppm Insert sample into the cuvette holder in the optical path and press ENTER to measure The...

Page 31: ...ve to highlight the test result and press to CLEAR DEL it Press PRINT to print the test results 2 4 DNA Protein There are three methods to choose for DNA Ratio RNA ratio and concentrations of RNA dsDN...

Page 32: ...Set Time Highlight Set Time Enter time in the order of hour minute and second Enter time in the order of hour minute and second For example 19 30 00 stands for 7 30pm 3 1 2 Set Date The date is enter...

Page 33: ...t to check and refresh the system dark current The marked 1 is the live dark current value at 0 gain which should not be zero or negative Press ENTER will refresh the dark current Press PRINT to view...

Page 34: ...stem Setup choose WL Calibration to recalibrate the system and the wavelength If you decide not to recalibrate the wavelength press ESC to return back to System Setup a Recheck Dark Current Dark curre...

Page 35: ...elength is pre calibrated and can be recalibrated using WL Calibration function If for any reason the wavelength accuracy is off it can be fine adjusted by reset it using the wavelength correction fun...

Page 36: ...ware Version You can check the firmware version from the System Setup 3 1 8 Lamp Service S2150UV ONLY This option is not available on S2150 Visible model At System Setup select Lamp Service and then c...

Page 37: ...manual 5 Accuracy Check 5 1 Wavelength Calibration Normally the S2150 Series spectrophotometer retains its wavelength calibration indefinitely However if the instrument receives a severe shock or is...

Page 38: ...step 6 until the wavelength setting reaches 370nm 8 Look for the maximum absorbance reading obtained and this should be found between 359 and 363nm 5 1 2 Didymium Filter Method 1 Set the Wavelength t...

Page 39: ...rs that might affect the results i e temperature bandpass weighing and diluting errors this method is less accurate and should only be used as a guide Reference Johnson E A Potassium Dichromate as an...

Page 40: ...e the lid The display should read 0 3 T Note It is recommended that you refresh the Dark Current before check 6 Lamp Replacement 6 1 Halogen Lamp Replacement Use screw drive to loosen M3 screws and re...

Page 41: ...rement Wrong wavelength setting Check sample procedure and wavelength setting Insufficient sample volume Fill cuvette with more sample solution Stray sample preparation vapors Prepare the sample away...

Page 42: ...42 Error Messages...

Page 43: ...and incorrect filter is brought into the optical path Sys energy low Pass system calibration and WL calibration but detects light beam energy low Energy to the detector is low The 0 order energy coun...

Page 44: ...r maybe loose 2 WL Opt coupler GK102 is malfunctioning 3 Light beam is misaligned or blocked failing to reach the detector 4 Lamp is off dead 5 Detector PCB malfunctioning dark current either negative...

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