8
6.2 Using the confocal microscope settings
6.2.1
Reusing settings from previous experiments
6.2.2
Setting up new experiment
6.2.3
Using Smart Setup
If you have earlier acquired
images with desired light paths,
open the image and use the
Reuse
button.
To create new light paths, you
can
Use the Smart Setup or create
them manually.
You can save a new imaging
configuration you created or
open an existing configuration
The
Smart Setup
function
allows you to insert your
fluorophores and the software
will
provide
light
path
suggestions.
Choose your fluorophore from
the dropdown box
Fastest
scans your labels with a
single scan. This gives the most
spectral
cross
talk/
bleed-
through and should be avoided.
Best signal
scans the light
paths separately and is the most
suitable option for everyday use.
Smartest (Line)
reduces the
number of scans and groups
together light paths, which
would produce least cross talk.
