4. Column Storage
Store the columns at constant temperature in the range of 4℃ to 30℃.
Don t store these columns below 4℃ not to freeze the solvent in the column.
Avoid exposing the columns to direct sunlight, and store the columns in a place safe
from corrosive gases.
If the column is being used daily, you need not remove the column from the
equipment and the buffer may be left in the column overnight, as long as the buffer
salt has good solubility. When the columns are to be stored for a few days, remove the
column from the instrument and seal both ends with the end plugs. For long-term
storage, the column must be protected from growth of microorganisms.
Thus, aqueous buffers should be relaced with distilled and deionized water.
5. Sample and Eluent Preparation
5-1 Replacement of Solvents
HIC type columns are filled with distilled and deionized water for shipment.
Replace this solvent with a suitable solvent, using one-half of the recommended flow
rate shown in Table 1.
Since frequent solvent replacement accelerates degradation of column efficiency, use
the same solvent as far as possible.
5-2 pH
These columns are usable over the relatively wide pH range of 2.0 - 12.0 at room
temperature.
5-3 Filtration
Use only HPLC grade solvents that have been filtered through an 0.5 μm filter.
Filter all buffered solution before using them. This reduces the problem of plugged
filters and preserves column life.
Vacuum filtration or sonification may be used to remove dissolved gasses which could
affect your solvent delivery system.
5-4 Salt Concentration Range
Salt concentration in the solvent is not limited up to near saturation.
− ３ −
