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Operation Manual

Thorlabs Instrumentation

LED Illumination System for Fluorescence Lifetime Imaging

(FLIM)

DC3100

2009

Summary of Contents for DC3100 Series

Page 1: ...Operation Manual Thorlabs Instrumentation LED Illumination System for Fluorescence Lifetime Imaging FLIM DC3100 2009 ...

Page 2: ...Version Date 1 3 04 09 2009 2009 Thorlabs ...

Page 3: ...nfiguration 3 1 3 3 1 3 1 15 User Limit 3 1 3 2 15 LED Configuration 3 1 3 3 16 System Settings 3 1 3 4 17 About 17 3 2 Remote Application 17 Installation 3 2 1 3 2 1 1 17 The Installation Menu 3 2 1 2 18 Installing VISA Runtime Engine 3 2 1 3 19 Installing the Remote Application 3 2 1 4 20 Driver Installation 20 Operating the DC3100 by the Remote Application 3 2 2 3 2 2 1 21 Connecting a Device 3...

Page 4: ... 37 7 2 Certifications and Compliances 39 7 3 Technical Data 39 Common Data 7 3 1 39 Technical Data 7 3 2 40 LED for Frequency Domain FLIM 7 3 3 7 3 3 1 40 LED 365nm 7 3 3 2 41 LED 405nm 7 3 3 3 42 LED 470nm 7 3 3 4 42 LED 630nm 44 7 4 Letter of Volatility 45 7 5 Thorlabs End of Life Policy WEEE 45 Waste Treatment on your own Responsibility 7 5 1 45 Ecological Background 7 5 2 46 7 6 List of Acron...

Page 5: ...ou Thorlabs GmbH This part of the instruction manual contains every specific information on how to handle and use the DC3100 FLIM LED Driver WARNING This symbol in the manual explains dangers that might result in personal injury or death Always read carefully the associated information before performing the indicated procedure ATTENTION This symbol in the manual explains hazards that could damage ...

Page 6: ...ord is correctly connected to the protective earth contact of the socket outlet Improper grounding can cause electric shock with damages to your health or even death The DC3100 must not be operated in explosion endangered environments The LED head control inputs and outputs must only be connected with duly shielded connection cables Only with written consent from Thorlabs may changes to single com...

Page 7: ...ue to the high intensity of the UV light If there is the possibility to receive a reflection of the UV light UV light protective glasses have to be used to prevent that UV light can catch one s eye directly If viewing the UV light is necessary use UV light protective glasses to avoid damage by the UV light CAUTION The head of the DC3100 is also a heat sink for the LED and will be heated up to more...

Page 8: ...hich can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures Reorient or relocate the receiving antenna Increase the separation between the equipment and receiver Connect the equipment into an outlet on a circuit different from that to which the receiver is connected Consult the dealer or an experience...

Page 9: ...ED Driver with LED Head 365nm DC3100 405 DC3100 FLIM LED Driver with LED Head 405nm DC3100 470 DC3100 FLIM LED Driver with LED Head 470nm DC3100 630 DC3100 FLIM LED Driver with LED Head 630nm Other wavelengths on request ranging from 365nm up to 940nm Please visit our homepage http www thorlabs com for further information ...

Page 10: ...ntry 3 1 Power supply 12V 3 75A 4 1 USB cable 5 1 Operation manual 6 1 Applications and driver CD 2 2 Preparation 1 Connect LED head cable with socket labeled LED at the back of the main control unit see figure 1 2 Connect power supply cable with main control unit use only the power supply supplied with your DC3100 unit 3 Plug in power supply Figure 1 Connecting the LED Head ATTENTION Prior to swi...

Page 11: ... screen for a few seconds The DC3100 is immediately ready to use after turning on The rated accuracy is reached however after a warming up period of approximately 10 minutes 2 3 Operating Elements 2 3 1 Operating Elements on the Front Panel Figure 2 Display and Operating Elements on the Front Panel ...

Page 12: ...2 Getting Started DC3100 10 2 3 2 Operating Elements on the Rear Panel Figure 3 Operating Elements on the Rear Panel 2 3 3 Operating Elements on the LED Head Figure 4 Operating Elements on the LED Head ...

Page 13: ...unctionality The button with LED indicator is used to switch the LED on or off The multi control is used to select the desired menu or to change the settings After switching on the DC3100 a Welcome screen appears for a few seconds Figure 5 Welcome Screen In case no LED head is connected you will be prompted to switch off the main unit connect the LED head and switch on the device The display will ...

Page 14: ...cessary The Ok button has no functionality within this mode The LED is switched on or off with the LED button The LED current can be changed when the LED is off as well as when it is in operation This allows to increase or reduce the brightness of the LED The current limit is displayed at the bottom of the screen Return to the main menu with the ESC button Figure 7 Constant Current Mode 3 1 2 2 In...

Page 15: ...he value will take effect immediately However since the editing mode is active the value has to be confirmed with the Ok button or can be canceled with the ESC button Figure 9 Internal Modulation Current Adjustment Figure 10 Internal Modulation Frequency Adjustment Figure 11 Internal Modulation Modulation Depth Adjustment See the chapter Technical Data for information about cut off frequencies for...

Page 16: ...y be controlled via the BNC connector at the rear panel of the DC3100 The applied voltage corresponds to the LED current 1V is equivalent to a LED current of 100mA A maximum voltage of 10V can be applied which results in a current of 1000mA Please note The maximum current is limited by the user defined current limit set in the Adjust Limit menu Figure 13 External Control Mode The Modulation Freque...

Page 17: ...lect an item with the multi control knob and press the Ok button to access the desired setting or configuration panel 3 1 3 1 User Limit The current limit can be changed by selecting User Limit The following screen appears and the LED current limit can be set using the multi control knob The new value has to be confirmed with the Ok button or canceled with the ESC button The LED current limit can ...

Page 18: ...6 LED Maximum Current Figure 17 LED Maximum Frequency ATTENTION A wrong and too high LED maximum current limit can cause damage to the LED Prior to changing the LED maximum current limit of the LED head check if the LED can handle this current 3 1 3 3 System Settings Within the system configuration the LCD backlight brightness can be set Select Configuration from the main menu via the multi contro...

Page 19: ...oftware can be used to operate the DC3100 FLIM LED Driver via the PC The device has to be connected to the PC by an USB cable 3 2 1 Installation 3 2 1 1 The Installation Menu Before installing the DC3100 remote application please make sure that no Thorlabs DC3100 FLIM LED Driver is connected After you inserted the DC3100 installation CD an autorun menu will appear If autorun is disabled on your sy...

Page 20: ...National Instruments VISA runtime engine provided on the installation CD You may also download the latest NI VISA runtime engine from the National Instruments web site www ni com visa In case you want to use the VISA engine provided on the installation CD select NI VISA Engine An installation wizard will be started If you are running Windows VISTA you might be prompted to change to the elevated mo...

Page 21: ...s After the DC3100 remote application installation wizard finished the initialization you will be prompted to specify the installation path Confirm with Next when you selected the installation path of your choice Follow the instruction of the installation wizard in order to install the remote software and drivers for the DC3100 During the installation process a separate installation wizard will st...

Page 22: ...Windows Update to search for Software First the USB to DC3100 Bridge will be installed 1 Please select No not this time and click Next to continue 2 Select Install the software automatically and click Next to continue 3 A message appears informing that the software has failed the Windows logo test Click Continue Anyway 4 Finalize the installation by clicking Finish After this first installation th...

Page 23: ... Select your DC3100 FLIM LED Driver by double clicking or pressing the Accept button The device will be connected and the last active mode will be entered The upload of the actual values on the system may take some seconds 3 2 2 2 Constant Current Mode The constant current can be selected by pressing the Constant Current Mode button see figure 35 or the corresponding menu entry Mode Constant Curre...

Page 24: ...the values directly by typing into the numeric controls or use the arrow controls The maximum user frequency is stored in the LED head and is specific to the mounted LED The maximum current is half of the user current limit which is set in the configuration menu Figure 26 The Internal Modulation Mode 3 2 2 4 External Control Mode The External Control Mode can be selected by pressing the External C...

Page 25: ... of the DC3100 The applied voltage corresponds to the LED current 1V is equivalent to a LED current of 100mA A maximum voltage of 10V can be applied which will result in a current of 1A Figure 27 The External Control Mode 3 2 2 5 User Limit Current The user can set an individual current limit which must be below the LEDs maximum current limit stored in the LED head Figure 28 User Limit ...

Page 26: ...2 2 6 Configuring a new LED The DC3100 FLIM LED Driver offers the possibility to operate a LED of your choice Use the menu Configuration LED Configuration to set the new LEDs maximum current and frequency Figure 30 LED Configuration Menu Figure 31 LED Maximum Limit ...

Page 27: ...Operating the DC3100 DC3100 25 Figure 32 LED Maximum Frequency 3 3 Changing the LED The DC3100 FLIM LED Driver offers the possibility to operate a LED of your choice Contact Thorlabs for more information ...

Page 28: ...ument and install the DC3100 drivers When the following message appears after the installation you have to install NI VISA from the distribution CD or from the National Instruments web site too Figure 33 No VISA Engine installed After successfully installing the software connect the DC3100 to a USB port of your PC The PC will find sequentially a DC3100 and a DFU device Please follow the instructio...

Page 29: ...rationMode Get Operation Mode DC3100_setLedOnOff Set LED OnOff DC3100_getLedOnOff Get LED OnOff DC3100_setModuCurrent Set Internal Modulation Current DC3100_getModuCurrent Get Internal Modulation Current DC3100_setModuFreq Set Internal Modulation Frequency DC3100_getModuFreq Get Internal Modulation Frequency DC3100_setModuDepth Set Internal Modulation Depth DC3100_getModuDepth Get Internal Modulat...

Page 30: ...rent limit in ampere Description Returns the current limit 4 3 1 1 5 Set Maximum Frequency Command DC3100_setMaxFrequency Parameter Maximum frequency in MHz Response None Description Sets the maximum frequency in MHz This limit should only be set in case a new LED was installed 4 3 1 1 6 Get Maximum Frequency Command DC3100_getMaxFrequency Parameter None Response Maximum frequency in MHz Descripti...

Page 31: ...lation current in Ampere Description Returns the current used for internal modulation in ampere 4 3 1 1 13 Set Internal Modulation Frequency Command DC3100_setModuFreq Parameter Frequency in MHz Response None Description Sets the internal modulation frequency in MHz 4 3 1 1 14 Get Internal Modulation Frequency Command DC3100_getModuFreq Parameter None Response Frequency in MHz Description Returns ...

Page 32: ... DC3100_getDispBright Parameter None Response Display brightness in percent Description Returns the display brightness 4 3 1 1 21 Get Status Register Command DC3100_getStatusRegister Parameter None Response Status register value Description Reads the content of the instruments status register Refer to chapter Status Reporting 4 3 1 1 22 Error Message Command DC3100_errorMessage Parameter Error cod...

Page 33: ... The LED head is not connected to the chassis Bit 2 VCC Fail Changed The bit VCC Fail has changed Bit 3 VCC Fail The power supply is out of range Bit 4 OTP Change The bit OTP Over Temperature has changed Bit 5 OTP Over temperature OTP in the chassis was detected The LED head was switched off Bit 6 LED Open Changed The bit LED Open has changed Bit 7 LED OPEN The LED head is connected but no LED was...

Page 34: ... file DC3100_Firmware_Update bat and the hex file DC3100 hex Both files have to be in the same directory Connect the DC3100 with a USB cable to your computer Check the COM port of the DC3100 It has to be COM1 COM2 COM3 or COM4 If not please change the COM port to one of the listed COM ports Before you switch on the DC3100 press the LED button and keep it pressed while you switch on the device The ...

Page 35: ...ched the maximum limit and the LED is switched off Make sure that the ventilation slots are not covered It is necessary to ensure an air ventilation through the DC3100 unit Software Installation failed The software cannot be installed on the computer Be sure to have administrative rights on your computer which enables you to install software at all Ask your system administrator to give you such ri...

Page 36: ...ncentration as well as further factors like molecular binding oxygen concentration or hydrophobic properties which will provide information about molecules of living cells The concentration of the dye the dimension of the cell or the excitation light intensity will not affect the lifetime Therefore the fluorescence lifetime imaging is a precise measure for ion concentration 6 1 1 Time Domain FLIM ...

Page 37: ...lates the fluorescence Due to the lifetime of the electrons in the excited state there will be a phase shift between the excitation signal and the fluorescence response Furthermore the intensity to the excitation and fluorescence sine waves will be modulated The lifetime can be calculated from the modulation ratio of the intensity Hence the FLIM in the frequency domain provides 2 values for the li...

Page 38: ...rants the hard and software determined by Thorlabs GmbH for this unit to operate fault free provided that they are handled according to our requirements However Thorlabs GmbH does not warrant a faulty free and uninterrupted operation of the unit of the soft or firmware for special applications nor this instruction manual to be error free Thorlabs GmbH is not liable for consequential damages Restri...

Page 39: ...ort Interruptions and Voltage Variations Immunity Performance Criterion A C 6 FCC EMC Compliance Emissions comply with the Class B Limits of FCC Code of Federal Regulations 47 Part 15 Subpart B 1 3 EC Declaration of Conformity Low Voltage Compliance was demonstrated to the following specification as listed in the Official Journal of the European Communities Low Voltage Directive 73 23 EEC amended ...

Page 40: ...ed the levels required by these standards may occur when this equipment is connected to a test object 5 MOD IN port capped at IEC 61000 4 3 test 6 Performance Criterion C was reached at additional test levels according to EN 61326 1 2006 table 2 ...

Page 41: ... x H x D with operating elements 160 x 80 x 168 mm 1 non condensing 7 3 2 Technical Data All technical data are valid at 23 5 C and 45 15 rel humidity LED Current Range 0 1000mA Modulation Frequency Range Internal Modulation Mode only 10 100MHz Modulation Internal Modulation Mode only Sine Wave Modulation Depth 1 Internal Modulation Mode only 0 100 Trigger Output Internal Modulation Mode only Sine...

Page 42: ...een reference output and the emitted light signal are shown 7 3 3 1 LED 365nm The DC3100 365 contains the UV LED NCSU033A from Nichia The LED emits a wavelength at 365nm and an optical power of up to 310mW Warning This DC3100 head radiates intense UV light during operation Precautions must be taken to prevent looking directly at the UV light with unaided eyes Do not look directly into the UV light...

Page 43: ...2 LED 405nm The DC3100 405 contains the UV LED LZ1 00UA05 from LedEngin The LED emits a wavelength at 405nm and an optical power of up to 1000mW The 10dB cut off frequency of the DC3100 405 is 95MHz The maximum current is 1000mA Figure 38 Phase Difference of DC3100 405 ...

Page 44: ...o 60lm The 10dB cut off frequency of the DC3100 470 is 80MHz The maximum current is 1000mA Figure 39 Phase Difference of DC3100 470 7 3 3 4 LED 630nm The DC3100 630 contains the red LED LZ1 00R105 from LedEngin The LED emits a wavelength at 630nm and an optical power of up to 100lm The 10dB cut off frequency of the DC3100 630 is 70MHz The maximum current is 1000mA ...

Page 45: ...7 Appendix DC3100 43 Figure 40 Phase Difference of DC3100 630 ...

Page 46: ...Program SRAM yes no Power down 128kByte FLASH ATmega1281 Program Code no no Cannot be cleared by user 4kbyte EEPROM ATmega1281 User settings no yes Can be overwritten with the device 1kByte EEPROM FTDI FT232R VID PID IO Configuration no no Cannot be cleared by user 256Byte SRAM FTDI FT232R USB receive buffer yes no Power down 128Byte SRAM FTDI FT232R USB transmit buffer yes no Power down ...

Page 47: ...M products that means assemblies to be built into a unit by the user e g OEM laser driver cards components mechanics and optics left over parts of units disassembled by the user PCB s housings etc If you wish to return a Thorlabs unit for waste recovery please contact Thorlabs or your nearest dealer for further information 7 5 1 Waste Treatment on your own Responsibility If you do not return an en...

Page 48: ...en Figure 6 Main Menu Figure 7 Constant Current Mode Figure 8 Internal Modulation Mode Figure 9 Internal Modulation Current Adjustment Figure 10 Internal Modulation Frequency Adjustment Figure 11 Internal Modulation Modulation Depth Adjustment Figure 12 Definition of the Parameter Current and Modulation Depth Figure 13 External Control Mode Figure 14 Main Menu Figure 15 LED Current Limit Setting F...

Page 49: ... under statutory regulations we assume no liability for direct damage indirect damage or damages suffered by third parties resulting from the purchase of this product In no event shall any liability exceed the purchase price of the product Please note that the content of this User Manual is neither part of any previous or existing agreement promise representation or legal relationship nor an alter...

Page 50: ...ales and Support Phone 1 973 579 7227 Fax 1 973 300 3600 Email sales thorlabs com Email techsupport thorlabs com Web www thorlabs com Japan Thorlabs Inc 1th Floor Higashi Ikebukuro Q Building 2 32 2 Higashi Ikebukuro Toshima ku Tokyo 170 0013 Japan Sales and Support Phone 81 3 5979 8889 Fax 81 3 5979 7285 Email sales thorlabs jp Web www thorlabs jp Our company is also represented by several distri...

Page 51: ... Mode 14 22 F Firmware Upgrade 32 Frequenca Domain FLIM 35 Front Panel 9 G General Information 4 I Internal Modulation Mode 12 22 L LED 365nm 40 LED 405nm 41 LED 470nm 42 LED 630nm 42 LED Configuration 15 LED Head 10 LEtter of Volatility 44 M Maintainance 32 Maximum Current 15 Maximum Frequency 15 Menus 11 N Navigation 11 O Operating Elements 9 10 Operation Modes 12 Ordering Codes 7 P Preparation ...

Page 52: ...Index DC3100 50 U Unpacking 8 User Limit 15 User Limit Current 23 Utility Software 26 V VISA Installation 18 W Warranty 36 WEEE 45 ...

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